人类β-防御素在口腔鳞癌组织中的表达及意义

目的 探讨人类β-防御素1（HBD-1）和人类β-防御素4(HBD-4)在口腔鳞癌中的表达及意义。方法 采用免疫组化SABC法,检测HBD1和HBD4在正常口腔组织、非典型增生组织和口腔鳞状细胞癌中的表达情况，并通过Image pro-plus 5.1图像分析软件对3组中HBD-1/HBD-4的染色进行平均吸光度值的测定。利用SPSS 16.0统计软件对各组数据进行单因素方差分析。结果 口腔黏膜中人类β-防御素1,4表达于增生鳞状上皮细胞和间质细胞和鳞状癌细胞中；HBD-1在正常口腔黏膜、白斑和口腔鳞癌组织中的表达呈增加趋势，但组间无显著差异（P>0.05）；HBD-4在正常口腔黏膜、白斑和口腔鳞癌组织表达呈明显增加趋势（P<0.01）。结论 HBD-1在口腔黏膜中呈固有表达，主要参与机体的天然免疫体系。HBD-4在口腔鳞状细胞癌的发生、发展中起重要的作用。

The expression and significance of human beta defensins in oral squamous cell carcinoma

Objective Discuss the expression and significance of human beta defensins（HBD-1 and HBD-4） in oral squamous cell carcinoma.Methods We adopted immunohistochemistry methods to detect different expressions of HBD-1 and HBD-4 in normal tissues,dysplasia tissues and oral squamous cell carcinoma.Image-plus 5.1 software was used to test the optical density of HBD-1and HBD-4 staining.The data were analyzed by one-way ANONA with SPSS 16.0 software.Results HBD-1 and HBD-4 expressed in the hyperplastic squamous epithelial cells,stromal cells and oral squamous cells in oral mucosa.The expression of HBD-1 in oral normal mucosa,leukoplakia and oral squamous cell carcinoma showed a strengthened trend,but no significant difference between groups existed（P0.05）.The expression of HBD-4 showed a significant increasing trend in the three groups（P0.01）.Conclusions These results suggest that HBD-1 may play an important role in innate defense.HBD-4 may play an important role in the development of oral squamous cell carcinoma.