| HIV—1gp41基因的分段克隆和表达 |
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| 引用本文： | 赵茜,徐志凯,阎岩,王海涛,吴兴安,张芳琳,刘勇,白文涛,罗雯膊,姜世勃.HIV—1gp41基因的分段克隆和表达[J].细胞与分子免疫学杂志,2001,17(5):484-485. |
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| 作者姓名： | 赵茜 徐志凯 阎岩 王海涛 吴兴安 张芳琳 刘勇 白文涛 罗雯膊 姜世勃 |
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| 作者单位： | 1. 第四军医大学微生物学教研室,
2. LFK Research Institute,New York Blood Center, |
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| 摘 要： | 目的 在大肠杆菌中表达HIV－1gp41N肽和C肽基因。方法 用PCR方法从含HIV－1gp160基因的质粒中扩增HIV－1gp41N肽和C肽基因，重组人pGEX-4T-1载体，并亚克隆人pGEM7zf( )中测定核苷酸序列，限制性酶切鉴定后进行原核表达。结果 成功地扩增到HIV－1gp41N肽和C肽基因，酶切鉴定及测序结果与已知HIV－1亚型的gp41N肽区和C肽区基因序列一致，SDS－PAGE结果显示，表达出与预期分子量大小相同的蛋白。结论 N肽和C肽基因的成功表达，为进一步研究其结构和功能奠定了基础。
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| 关 键 词： | HIV－1 gp41 基因扩增 原核表达 |
| 文章编号： | 1007-8738(2001)05-484-02 |
| 修稿时间： | 2001年5月30日 |
Cloning and expressing of HIV-1 gp41 N and C peptide gene |
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| ZHAO Qian ,XU Zhi -kai ,YAN Yan ,WANG Hai -tao ,WU Xing -an ,ZHANG Fang -lin ,LIU Yong ,BAI Wen -tao ,LUO Wen ,JIANG Shi -bo.Cloning and expressing of HIV-1 gp41 N and C peptide gene[J].Journal of Cellular and Molecular Immunology,2001,17(5):484-485. |
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| Authors: | ZHAO Qian XU Zhi -kai YAN Yan WANG Hai -tao WU Xing -an ZHANG Fang -lin LIU Yong BAI Wen -tao LUO Wen JIANG Shi -bo |
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| Institution: | ZHAO Qian 1,XU Zhi -kai 1,YAN Yan 1,WANG Hai -tao 1,WU Xing -an 1,ZHANG Fang -lin 1,LIU Yong 1,BAI Wen -tao 1,LUO Wen 1,JIANG Shi -bo 21 Department of Microbiology,Fourth Military Medica |
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| Abstract: | Aim To express N and C peptide genes of HIV-1gp41in E.Coli JM109.Methods N and C peptide genes of HIV -1gp41were amplified from plasmid containing c DNA of HIV -1gp160gene by PCR and cloned into prokaryotic expression vector pGEx-4T -1.The cloned genes were subcloned into pGEM7zf( )and sequenced.Expression of the cloned genes were induced in E.coli JM109and analysed on SDS -PAGE.Results The N and C peptide genes were amplified and cloned successfully,and which was confirm ed by restriction endonuclease and sequence analysis.The fused GST -N a nd GST -C were expressed successfully identified by SDS -PAGE.Conclusion The expression of N and C peptide genes of HIV -1gp41have laid the foundation for further investigation. |
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| Keywords: | HIV -1 gp41 gene amplification e ukaryocyte expression |
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