毛发中度冷丁及其代谢产物的鉴定

目的：为考察度冷丁滥用者毛发中度冷丁及其代谢物的存在状况，建立GC/MS和GC/NPD测定人毛发中度冷丁及代谢产物去甲度冷丁的定性定量分析方法。方法：毛发经酸水解后用乙醚直接提取，GC/MS和GC/NPD测定。结果：度冷丁及去甲度冷丁回收率>80%，最低检出限分别为0.5 ng.mg^-1和1 ng.mg^-1。在度冷丁滥用者毛发中鉴定、确认度冷丁及其代谢物去甲度冷丁、乙酰去甲度冷丁，并测定了50例度冷丁滥用者毛发中度冷丁及去甲度冷丁的浓度，分别为4.8～496 ng.mg^-1和15.5～685 ng.mg^-1。结论：方法简便、可靠、灵敏，可作为法医鉴定和临床诊断的工具。

DETECTING MEPERIDINE AND ITS METABOLITE NORMEPERIDINE IN HUMAN HAIR

AIM: To identify meperidine and its metabolites normeperidine in hair of meperidine addicts and establish the GC/MS and GC/NPD methods for determination of meperidine and normeperidine in human hair . METHODS: 5～10 mg washed hair was cut into small pieces, after hydrolysis, meperidine and normeperidine were extracted with diethyl ether and determined by GC/MS and GC/NPD. The recoveries of meperidine and normeperidine were greater than 80%, and the lower limits of detection were 0.5 ng.mg^-1 and 1 ng.mg^-1 respectively. RESULTS: Meperidine and its metabolites normeperidine and normeperidine-AC were found by GC/MS (EI, PCI) in the hair of meperidine addicts, and the hair contents of meperidine and normeperidine of 50 drug abusers were 4.8～496 ng.mg^-1 and 15.5～685 ng.mg^-1, respectively. CONCLUSION: The methods are simple, accurate and sensitive. These are adopted in routine analysis for confirmation of meperidine-abuse cases.