Exposure of cdc8 homozygous diploids of Saccharomyces cerevisiae to nonpermissive temperature leads to an increase in mitotic conversion frequencies |
| |
Authors: | Hanna Baranowska Daniela Zaborowska Jerzy Żuk |
| |
Institution: | (1) Polish Academy of Sciences, Institute of Biochemistry and Biophysics, Rakowiecka 36, 02-532 Warsaw, Poland |
| |
Abstract: | Summary In a diploid strain homozygous for the cdc8-1 mutation, a block in DNA synthesis caused by restrictive temperature resulted in a significant increase in the frequency of intragenic recombination at the HOM2 locus. Under restrictive conditions, incorporation of radioactivity into DNA was reduced to 2% of the control and alkaline sucrose gradient centrifugation revealed that only short DNA fragments were synthesized. There was no considerable fragmentation of template DNA during incubation of cdc8-1 strains under restrictive conditions. |
| |
Keywords: | Yeast cdc8-1 mutation Mitotic recombination |
本文献已被 SpringerLink 等数据库收录! |