| 大鼠弥散性脑损伤hsp70 mRNA表达的研究 |
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| 引用本文： | 王晔,潘洪富,张夔鸣,陈晓刚,彭其毅,王文东,刘敏. 大鼠弥散性脑损伤hsp70 mRNA表达的研究[J]. 四川大学学报(医学版), 2004, 35(6): 828-831 |
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| 作者姓名： | 王晔 潘洪富 张夔鸣 陈晓刚 彭其毅 王文东 刘敏 |
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| 作者单位： | 1. 四川大学华西基础医学与法医学院,法医病理学教研室,成都,610041
2. 四川大学华西公共卫生学院 |
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| 摘 要： | 目的寻找脑损伤早期诊断指标。方法复制大鼠弥漫性脑损伤模型，用原位杂交和图象分析技术检测不同损伤时间的大鼠恼干、丘脑、大脑皮层等部位神经元和神经胶质细胞中hsp70 mRNA表达情况。结果发现不同损伤时间，hsp70 mRNA表达的强弱不同；在损伤5min后即可在脑干、丘脑、皮质等部位检测到hsp70 mRNA，6h达高峰，12h后减弱。结论hsp70 mRNA可以作为早期脑弥散性损伤的指标，并可用于生前伤与死后伤的判定。
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| 关 键 词： | 原位杂交 hsp70mRNA 弥散性脑损伤 |
| 修稿时间： | 2003-11-07 |
A Study on Expression of hsp70 mRNA in the Early Stage of Diffuse Brain Injury in Rats |
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| Ye Wang,Hong-fu Pan,Kui-ming Zhang,Xiao-gang Chen,Qi-yi Peng,Wen-dong Wang,Min Liu. A Study on Expression of hsp70 mRNA in the Early Stage of Diffuse Brain Injury in Rats[J]. Journal of Sichuan University. Medical science edition, 2004, 35(6): 828-831 |
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| Authors: | Ye Wang Hong-fu Pan Kui-ming Zhang Xiao-gang Chen Qi-yi Peng Wen-dong Wang Min Liu |
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| Affiliation: | Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. |
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| Abstract: | Objective To evaluate the changes of hsp70 mRNA in neuron and glia of rat suffering early diffuse brain injury. Methods SD rats were used in the replication of the animal pattern of diffuse brain injury. In situ hybridization technique and image analysis technique were applied in detecting the hsp70 mRNA in the rat's cerebral cortex, thalamus and brain stem. Results The signal intensity of hsp70 mRNA could be detected in 5 minutes after brain injury; subsequently, it attained to the summit at 6 hours and declined at 12 hours after brain injury. Conclusion The above data suggest that hsp70 mRNA is useful for the diagnosis of early diffuse brain injury and for distinguishing the postmortern injury from antemortem injury. |
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| Keywords: | in situ hybridization hsp70 mRNA Diffuse brain injury |
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