加味消渴康对糖尿病肾病大鼠肝组织细胞外基质相关调节因子表达的影响

目的基于中医＂肝肾同源＂理论,观察加味消渴康对糖尿病肾病大鼠肝组织转化生长因子β1（transforming growth factor-β1,TGF-β1）,Smad-4蛋白（drosophila mothers against decapentaplegic protein-4）,基质金属蛋白酶9（matrix metalloproteinase-9,MMP-9）和金属蛋白酶组织抑制剂1（tissue inhibitor of metalloproteinases-1,TIMP-1）表达的影响。方法采用单侧肾切除加链脲佐菌素诱导的糖尿病肾病模型,将造模成功后的50只糖尿病肾病大鼠随机分为模型组、西药组和中药小、中、大剂量组,每组10只,另选取10只正常大鼠作为正常对照。其中西药组予氯沙坦（5.2 mg/kg·d）灌胃,中药组予加味消渴康灌胃（小、中、大剂量分别为13.125 g/kg·d,26.25 g/kg·d和52.5 g/kg·d）,正常组及模型组每日灌服2 ml蒸馏水,共灌胃12周。各组大鼠于第12周末处死取出肝脏,运用ELisa法观察大鼠肝组织TGFβ1、Smad-4、MMP-9和TIMP-1的表达。结果与正常组相比,各组大鼠肝中TGF-β1,Smad-4和TIMP-1表达明显升高,而MMP-9的表达明显降低,差异具有统计学意义（P〈0.05或P〈0.01）,中药治疗后,各组大鼠TGF-β1,Smad-4的表达与模型组相比明显降低,差异具有统计学意义（P〈0.01）,而TIMP-1和MMP-9的表达则分别具有降低和升高的趋势。结论加味消渴康对DN大鼠的肝损伤具有一定的保护作用。

The effects of JiaweiXiaokekang on the expressions of factors regulating the hepatic extracellular matrix in DN rats

Objective To investigate the influence of JiaweiXiaokekang on the expression of TGF-β1,Smad-4,MMP-9 and TIMP-1 in the hepatic tissues of diabetic nephropathy（ DN） rats based on the ＂ theory of homogeny of liver and kidney＂. Methods The rat model was established by uninephrectomy and injection of streptozotocin. 50 models were divided into 5 groups： model group,western medicine group and Chinese herb medicine groups including low-dose,medial-dose and high-dose group. There were 10 models in each group. Besides,another 10 normal rats were chosen as the normal group. Western medicine group was given losartan（ 5. 2 mg / kg·d） by intragastric administration; Chinese herb medicine gro ups were given JiaweiXiaokekang（ low-dose group： 13. 125 g / kg·d,medial-dose group： 26. 25 g / kg·d and high-dose group： 52. 5 g / kg·d-1） by intragastric administration; The normal group and model group were fed with 2 ml distilled water daily. After 12 weeks,sacrificed all the rats and removed their hepars and then observed the expressions of TGF-β1,Smad-4,MMP-9 and TIMP-1 by immunohistochemistry. Results Compared with the normal group,the expression of TGF-β1,Smad-4 and TIMP-1 in other groups significantly increased,while the expression of MMP-9 significantly decreased（ P ＆lt; 0. 05 or P ＆lt; 0. 01）. After the treatment,compared with the model group,the expression of TGF-β1 and Smad-4 in other groups significantly decreased. While there was a decreased or increased trend on the expression of TIMP-1 and MMP-9 respectively. Conclusion JiaweiXiaokekang had a certain protective effect on the hepatic injury of DN rats.