铁代谢红系调节因子在不同类型红系造血异常疾病中的表达

目的:研究Erythroferrone（ERFE）等铁代谢红系调节因子（iron-regulatory erythroid factor）在不同类型红系造血异常疾病中的表达情况。方法:采用ELISA方法检测2016年1月至2019年11月共47例真性红细胞增多症（PV）、纯红细胞再生障碍（PRCA）、自身免疫性溶血性贫...

Expression of iron-regulating erythroid factors in different types of erythropoiesis disorders

Objective To investigate the expression of iron-regulating erythroid factors in different types of erythropoiesis disorders.Methods From January 2016 to November 2019,the plasma concentrations of iron-regulating erythroid factors were measured by ELISA methods in 47 patients with different types of erythropoiesis disorders.The adaptation orientation of iron-regulating erythroid factor expression with bone marrow erythropoiesis activities(represented by bone marrow-nucleated erythrocytes ratio)was analyzed.Results The median plasma growth differentiation factor(GDF)15 levels in patients with polycythemia vera(PV),pure red cell aplasia(PRCA),autoimmune hemolytic anemia(AIHA),and myelodysplastic syndrome(MDS)were 266.01 ng/L(112.40,452.37),110.63 ng/L(81.41,220.42),52.11 ng/L(32.61,171.66),and 276.53(132.16,525.70)ng/L,respectively,which were significantly higher than those in normal patients with 37.45(19.65,57.72)ng/L(all P<0.01).The plasma TWSG1 expression levels were not significantly different in patients with PV,PRCA,AIHA,and MDS from those of normal patients(P>0.05).The median plasma GDF11 level in PV was 74.75(10.95,121.32)ng/L,which was significantly higher than 36.90(3.38,98.34)ng/L in normal control subjects(P<0.01).However,no statistical differences were observed in the other three subjects(P>0.05).The median plasma erythroferrone(ERFE)levels in AIHA and PV were 121.76 ng/L(68.12,343.11)and 129.63(47.02,170.03)ng/L,respectively,with the highest level in AIHA in all the studied types of erythropoiesis disorders.The bone marrow-nucleated erythrocytes ratio was significantly and positively correlated with ERFE(r=0.458,P=0.001)but not with GDF15(r=-0.163,P=0.274),GDF11(r=0.120,P=0.421),and TWSG1(r=-0.166,P=0.269).Conclusion The expression profile of iron-regulating erythroid factors is not exactly the same in different types of erythropoiesis disorders.ERFE demonstrated the highest correlation with erythropoiesis activities.