肉苁蓉与沙苁蓉中6种苯乙醇苷类成分的UPLC含量测定及化学计量学评价

目的:建立肉苁蓉、管花肉苁蓉及沙苁蓉中松果菊苷、管花苷A、毛蕊花糖苷、异毛蕊花糖苷、金石蚕苷和2'-乙酰金石蚕苷的UPLC含量测定方法,并比较其含量的异同.方法:采用Waters HSST3(100 mmx 2.1 mm,1.8 μm)色谱柱,流动相为甲醇-0.1％甲酸水,梯度洗脱,流速0.3 mL·min-1,检测波...

Determination and chemometric evaluation of six phenylethanolic glycosides in Cistanche deserticola and Cistanche sinensis by UPLC

Objective:To establish a UPLC method for the determination of echinacoside,cistanoside A,acteoside,isoacteoside,poliumoside and 2′-acetylpoliumoside in Cistanche deserticola,Cistanche tubulosa and Cistanche sinensis,and compare their contents.Methods:UPLC analysis was performed on Waters HSS T3(2.1 mm×100 mm,1.8μm)with a mobile phase consisting of methanol-0.1%formic acid aqueous with gradient eluent at a flow rate of 0.3 m L·min^-1 and the injection volume was 1μL.The detection wavelength was 330 nm and the column temperature was 30℃.The data were evaluated by chemometrics.The six components were determined.Results:Under the above conditions,the linear relationship for six components were separated well and in the ranges of 30.80-1232μg·mL^-1,0.7980-31.91μg·mL^-1,3.310-132.4μg·mL^-1,0.933-37.32μg·mL^-1,59.02-885.3μg·mL^-1 and 35.73-536.0μg·mL^-1,respectively,with good linear relationship r≥0.9999.The average recoveries(n=6)were 98.5%,97.3%,98.7%,99.3%,99.7%and 98.4%,respectively.The RSDs of precision,repeatability and stability tests were<4.0%.Poliumoside and 2’-acetylpoliumoside were the specific components of Cistanche sinensis,while acteoside was the common component of Cistanche deserticola,Cistanche tubulosa and Cistanche sinensis,but the species and content of phenylethanolic glycosides in Cistanche sinensis were different from those of Cistanche deserticola and Cistanche tubulosa.The principal component analysis could distinguish the genuine Cistanche deserticola from the fake Cistanche sinensis.Conclusion:The method is simple,accurate and reproducible,which can provide reference for quality evaluation and interspecific identification of Cistanche deserticola,Cistanche tubulosa and Cistanche sinensis.