Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
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| Authors: | Hung-Chang Hung Chi-Ho Chan Shih-Ming Tsao Shiuan-Chih Chen Tzu-Chin Wu Min-Chi Lu Der-Jinn Wu Shu-Ju Huang Yuan-Ti Lee |
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| Institution: | 1. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;2. Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan;3. Potz Hospital, Department of Health, Executive Yuan, Chiayi, Taiwan;4. Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan;5. Division of Chest, Department of Internal Medicine, Chung Shan Medical University, Taichung, Taiwan;6. Division of Infectious Diseases, Department of Internal Medicine, Chung Shan Medical University, Taichung, Taiwan;7. Department of Family and Community Medicine, Chung Shan Medical University, Taichung, Taiwan;8. Division of Cardiology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan |
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| Abstract: | ObjectiveThe diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy.MethodAFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards.ResultsThe sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively). The sensitivity and specificity of the DTB assay in detecting MTB in sputum samples was 77.2% and 100% compared to clinical diagnosis, while AFB smear had a sensitivity and specificity of 70.3% and 26.3%, respectively. Compared to culture, DTB had a sensitivity and specificity of 82.8% and 93.2%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 41.9% and 87.7%, respectively. Compared to clinical diagnosis, DTB had a sensitivity and specificity of 67.2% and 100%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 34.8% and 79.5%, respectively.ConclusionsThe superior performance of the DTB assay relative to AFB smear microscopy makes it a valuable tool to enable early diagnosis of MTB, thereby improving patient care and reducing transmission. |
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