辐射诱发胸腺淋巴瘤与Ikaros及p16基因多态性

目的 探讨Ikaros及p16基因多态性与辐射诱发小鼠胸腺淋巴瘤的相关性。方法 采用Prom ega公司基因组DNA纯化试剂盒提取胸腺淋巴瘤细胞基因组DNA。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测Ikaros基因rs28185870位点(C/T)、rs28185923位点(C/T)及p16基因rs3695947位点(A/G)单核苷酸多态性(SNPs)。结果 C57BL/6 J及BALB/c小鼠rs28185870位点基因型分别为T/T、C/C;rs28185923位点基因型分别为C/C、T/T;rs3695947位点基因型分别为A/A、G/G。结论 辐射敏感性不同的C57BL/6 J、BALB/c小鼠的rs28185870、rs28185923及rs3695947位点基因型不同,推测Ikaros及p16基因多态性与辐射诱发胸腺淋巴瘤相关。

Association between polymorphism of Ikaros and p16 genes and thymic lymphoma induced by Ionizing radiation in mice

Objective To investigate the association between thymic lymphom a induced by ionizing radiation and the polymorphism of Ikaros and p16 genes in mice.Methods Genomic DNA of thymic lymphoma cells was extracted by genomic DNA purification kit.PCR-restriction fragment length polym orphism(RFLP)was used to examine the single nucleotide polymorphisms(SNPs)of rs28185870(C/T)and rs28185923(C/T)of Ikaros gene and rs3695947(A/G)of p16 gene.Results The genotype of rs28185870 site in C57BL/6J and BALB/c was T/T and C/C.The genotype of rs28185923 site was C/C and T/T.The genotype of rs3695947 site was A/A and G/G.Conclusion The genotypes of rs28185870,rs28185923 and rs3695947 in C57BL/6J and BAL B/c mice with different radiosusceptibility were different.It is suggested that there is an association between the polymorphism of Ikaros andp16 genes and thymic lymphom a induced by ionizing radiation.