大鼠复合内毒素血症致肺微循环变化与肺泡表面活性物质功能的关系

本实验应用大鼠烫伤复合内毒素血症的模型,制备肺组织超薄切片,进行透射电镜观察,同时测定其肺泡表面活性物质的功能;结合白细胞计数、血清和肺组织内二醛(MDA)含量以及超氧化物歧化酶(SOD)活性的测定。结果显示,肺毛细血管内粒细胞增多并粘附于内皮细胞膜;内皮细胞受损。肺泡表面活性物质功能明显降低。血清和肺组织MDA含量明显增加,SOD活性则显著下降。用SOD治疗能明显改善上述病变。本实验结果提示,肺微循环障碍是烫伤复合内毒素血症致急性肺损伤时肺泡表面活性物质功能下降的病理基础,与白细胞在肺血管内陷落、激活、释放自由基有关。SOD对此有一定的保护作用。

Relationship of Pulmonary Microcirculation and Function of PS in Acute Lung Injury Induced by Burns Companied with Endotoxemin in Rats.

An animal model of acute lung injury was produced by intravenous injection of E. coli endotoxin to burned rats. The structural changes of lung were studied using transmission electron microscope. The function of PS and counts of WBC were measured simultaneously. The MDA level and SOD activity in the plasma and lung hemofenate were measured at the same time. The results showed that the leukocytes accumulated in the pulmonary capillaries and adhered to endothelia. There was distruption of the endothelia cell layer. The function of PS decreased, level of MDA increased and SOD activity decreased significantly. This study suggests that pulmonary microcirculatory changes are the pathological basis of decreas in PS function. It has relation to accumulation, activited and release of oxygen radicals of leukocytes in pulmonary capillaries. SOD can improve the changes mentioned above.