| 细胞周期与组织工程 |
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| 引用本文： | 王向辉,毛津淑,崔元璐,姚康德. 细胞周期与组织工程[J]. 中国修复重建外科杂志, 2003, 17(2): 173-176 |
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| 作者姓名： | 王向辉 毛津淑 崔元璐 姚康德 |
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| 作者单位： | 天津大学材料学院高分子材料研究所,天津,300072 |
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| 基金项目： | 国家重点基础研究发展规划项目 (973) :组织工程的基本问题 (G1 9990 5430 5)，国家自然科学基金资助项目 (598830 0 2 )~~ |
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| 摘 要： | 目的 研究细胞周期在组织工程研究中的应用，为材料的生物相容性评价提供一种新思路。方法 利用免疫细胞化学，蛋白质印迹法，RT—PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。结果 通过研究材料和细胞的相互作用，表明材料的拓扑结构、物理和化学结构都会影响吸附在材料上的细胞的细胞周期。并且会影响与细胞周期、增殖和凋亡相关基因的表达。结论 细胞周期的研究能够综合反映出细胞增殖、分化及凋亡等生理活动的动态平衡，有希望成为生物相容性研究的一种重要方法。
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| 关 键 词： | 细胞周期 组织工程 生物相容性 免疫细胞化学 蛋白质印迹法 基因表达 生物材料 |
| 修稿时间： | 2001-12-06 |
CELL CYCLE AND TISSUE ENGINEERING |
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| WANG Xiang hui,MAO Jin shu,CUI Yuan lu,et al.. CELL CYCLE AND TISSUE ENGINEERING[J]. Chinese journal of reparative and reconstructive surgery, 2003, 17(2): 173-176 |
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| Authors: | WANG Xiang hui MAO Jin shu CUI Yuan lu et al. |
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| Affiliation: | WANG Xiang hui,MAO Jin shu,CUI Yuan lu,et al. Research Institute of Polymeric Materials,Tianjin Universiyt. Tianjin,P.R.China 300072 |
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| Abstract: | Objective To investigate cell cycle as a new tool to evaluate the biocompatibility of biomaterials.Methods The cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry. Results The physical properteis, chemical properties and topological properities of biomaterials could not only influence cell cycle of the cells attached onto biomaterials but also affect the expression of related genes of target cells. Conclusion As an important extension of routine proliferation epxeriments, the study of cell cycle control will be great help for us to study the cell group as an organic society. It revealed the balance between cell proliferation, cell differentiation and apoptosis. It is suggested that the study of cell cycle control will play a key role in the research of tissue engineering. |
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| Keywords: | Tissue engineering Biomaterials Cell cycle Apoptosis |
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