日本血吸虫感染小鼠组织内的抗原,抗体定位

应用间接荧光抗体试验(IFAT)检测小鼠感染血吸虫后肝、肠内抗原,结果感染后4wk未检出抗原,6wk抗原水平显著升高,10—12wk达到高峰、其几何均数倒数分别高达512和483。组织内虫卵肉芽肿的病变,亦最为显著,而肝内虫卵肉芽肿的直径显著大于肠内的。用直接荧光抗体试验(DFAT)及IFAT测定小鼠感染血吸虫后肝、肠组织内抗体,结果在感染6wk后,三种抗体(IgG、IgM和IgA)均达低水平,10—12wk均显著上升,而肝、肠组织内IgG抗体则非常显著地高于IgM和IgA的。提示血吸虫虫卵肉芽肿的免疫应答,主要是IgG抗体。

LOCALIZATION OF ANTIGEN AND ANTIBODIES IN THE LIVER AND INTESTINE OF MICE WITH SCHISTOSOMA JAPONICUM

Indirect fluorescent antibody test was used to localize the antigen in the livers and intestines of mice with Schistosoma japonicum. No antigen was detected 4 weeks p. i.. There was a significant rise in antigen level 6 weeks p. i. which reached peak level 10-12 weeks p. i., the GMRT being 512 and 483, respsectively. The eggs in the tissues showed strong fluorescent reaction 6 weeks p. i.. The mean diameter of schistosome egg granulomas in the livers and intestines of infected mice was remarkably enlarged 6 weeks after infection. The mean diameter of egg granulomas in the livers was markedly larger than that in the intestines. The antibodies were localized in the livers and intestines of infected mice as show en by the direct fluorescent antibody test (DFAT) and IF AT. The results indicated that the levels of IgG, IgM and IgA antibodies appeared 6 weeks p. i. which went on a significant increase at the 10-12weeks. The IgG antibody level was higher than that of other two antibodies. It is suggested that the IgG may be the main antibody in the immunologic response of Sj egg granulomas.