间日疟原虫卵囊的发育和子孢子形成的透射电镜观察

间日疟原虫卵囊的发育以囊内细胞质出现空泡、裂隙开始,裂隙将细胞质分割成多个成孢子细胞。子孢子形成以成孢子细胞表膜下致密内膜和膜下微管形成开始,继之形成顶端复合物,随着突起增大,表膜复合膜从突起顶部向后延伸,最后包绕整个子孢子。子孢子膜下微管为10-12个,微管的排列方式不一。 疟原虫生活史中第二次减数分裂可能发生在子孢子形成期间。

ULTRASTRUCTURAL OBSERVATION ON THE OOCYST DEVELOPMEN T AND SPOROZOITE FORMATION OF PLASMODIVM VIVAX

The oocyst development of Plasmodium vivax was heralded by the appearance of cyto-plasmic vacuoles and membraneous clefts. These clefts broke up oocyst cytoplasm into many sporoblasts. The onset of transformation from oocyst to sporozoite was heralded by forming pellicular complex, at some point of pelliculum of sporoblast dense inner membrane and subpellicular microtubules formed beneath the plasmalemma. The pellicular complex bulged out to form sporozoite buds appearing as small domes. Following enlargement of sporozoite buds the apical ends of the buds became conical in longitudinal sections with the newly formed apical rings at their truncated apices and then, newly formed dense inner membranes and subpellicular microtubules gradually extended backwards and finally enclosed the sporozoi-tes. Beneath the inner membrane there were a total of 10-12 subpellicular microtubules arranged irregularly as 5 6, 6 5, 6 6, 7 4, 8 3, 10 1 and 11 1.During the formation of sporozoite, intranuclear spindle appeared in the nucleus, suggest ting that the second meiosis in the life cycle of Plasmodium vivax occurred in developing sporozoites (Figs. 1-16).