Survivin mRNA、Caspase-3mRNA在舌鳞癌中的表达及相关性

目的:检测舌鳞癌组织中凋亡抑制基因Survivin mRNA及Caspase-3 mRNA的表达,探讨其在舌鳞状细胞癌中的意义及相关性。方法:应用原位杂交方法检测10例正常舌黏膜、45例舌鳞状细胞癌标本中Survivin mRNA及Caspase-3 mRNA的表达情况。采用SPSS13.0软件包进行χ2检验及Fisher精确概率法检验,指标间关系采用Spearman等级相关分析。结果:舌鳞癌标本中Survivin mRNA阳性表达率为55.6%,正常对照组全部为阴性,两组差异显著(P<0.01);舌鳞癌标本中Caspase-3 mRNA阳性表达率为42.2%,明显低于正常对照组的80.0%(P<0.05);Survivin mRNA高表达及Caspase-3 mRNA低表达与舌鳞癌组织学分级、颈淋巴结转移相关(P<0.05);与TNM分期密切相关(P<0.01);经Spearman等级相关分析,两者之间呈显著负相关,r=-0.412,P=0.005。结论:在舌鳞癌中,Survivin mRNA参与了抑制Caspase-3m RNA的表达,且这种抑制作用与肿瘤的发生、发展相关。

Expression of Survivin mRNA and Caspase-3 mRNA and their correlation in tongue squamous cell carcinoma

PURPOSE:To investigate the expression of Survivin mRNA and Caspase-3 mRNA in tongue squamous cell carcinoma(TSCC),and clarify their significance and correlation in TSCC.METHODS:In situ hybridization technique was employed to detect the expression of Survivin mRNA and Caspase-3 mRNA in 10 normal lingual mucous membranes(NLMM)and 45 TSCCs.Data was analyzed with SPSS13.0 software package for Chi-square test and Fisher's exact test,and Spearman rank correlation was used for analysis of their correlation.RESULTS:The positive staining rate of Survivin mRNA in TSCC was 55.6%,and significantly higher than that in normal control group(P<0.01).The positive staining rate of Caspase-3 mRNA in TSCC was 42.2%,and significantly lower than that in normal control group 80.0%(P<0.05).Overexpression of Survivin mRNA and underexpression of Caspase-3 mRNA were related to the pathological grade,cervical lymphatic metastasis of TSCC(P<0.05)and closely related to TNM stage(P<0.01).There was a negative relation between them,and the Spearman correlation coefficient r was-0.412,P=0.005.CONCLUSIONS:The higher expression of Survivin mRNA takes part in inhibiting Caspase-3 mRNA expression,which correlates with carcinogenesis and progress of TSCC.Supported by Science and Technology Research Project of Liaoning Province(Grant No.2005225007-1).