| 高效液相色谱法测定人血浆中舍曲林浓度 |
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| 引用本文： | 刘文宪,陈清霞,刘伟忠,温预关.高效液相色谱法测定人血浆中舍曲林浓度[J].海峡药学,2011,23(9):208-210. |
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| 作者姓名： | 刘文宪 陈清霞 刘伟忠 温预关 |
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| 作者单位： | 1. 广州市脑科医院国家药品临床研究基地,广州,510370
2. 中山大学附属第二医院药学部,广州,510120 |
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| 基金项目： | 广东省自然科学基金资助项目 |
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| 摘 要： | 目的 建立测定人血浆中舍曲林浓度的高效液相色谱法.方法 以Diamonsil(TM)C18反相柱(150mm×4.6mm,5μm)为色谱柱,流动相为0.03mol·L-1醋酸铵-甲醇(24:76);流速:0.8mL·min-1;柱温:40℃检测波长:210nm.以乙酸乙酯与氯甲烷(80:20)为提取剂.结果 舍曲林的低...
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| 关 键 词： | 舍曲林 血药浓度 高效液相色谱法 |
Determination of the concentration of sertraline in human plasma by HPLC |
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| LIU Wen-xin,CHEN Qing-xia,LIU Wei-zhong,WEN Yu-guan.Determination of the concentration of sertraline in human plasma by HPLC[J].Strait Pharmaceutical Journal,2011,23(9):208-210. |
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| Authors: | LIU Wen-xin CHEN Qing-xia LIU Wei-zhong WEN Yu-guan |
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| Institution: | LIU Wen-xin1,CHEN Qing-xia2,LIU Wei-zhong1,WEN Yu-guan1(1.Base of Drug Clinical Study of Guangzhou Brain Hospital,Guangzhou 510370,China,2.Department of pharmicy,The Second Affiliated Hospital,Sun Yat-sen University,Guangzhou 510120,China) |
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| Abstract: | OBJECTIVE To establish a method for determining the concentration of sertraline in human plasma by HPLC.METHODS The drug from plasma was analyzed in a reverse phase HPLC system C18 column(150mm×4.6mm,5μm);mobile phase consisted of 0.03 mol·L-1 ammonioum-methanol(24∶76);the flow rate was 0.8mL·min-1;the detection wavelength was at 210nm.Ethyl acetate and dichloromethane(80∶20) was used as extracting solvent.RESULTS The average recoveries of sertraline in low,middle and high concentrations(20.0,80.0,320.0ng·mL-1) were 100.78%,99.13%and 98.86%,respectively.The intra-day and inter-day variation(RSD) was less than 9%(n=5).The calibration curve of sertraline showed good linearity,r=0.9986(n=8),over the range of 10.0～480.0ng·mL-1.The regression equation was C=87.62F+1.37.r=0.9989(n=8).The minimum detectable concentration of sertraline was 5.0ng·mL-1.CONCLUSION The method is sensitive,accurate,simple and quick,it can be used for clinical drug monitoring and pharmacokinetics studies of sertraline. |
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| Keywords: | Sertraline Plasma concentration HPLC |
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