中药循经敷贴法对豚鼠哮喘模型的影响

目的:观察中药循经敷贴法对豚鼠哮喘潜伏期的影响,以及含药血清对抗氯化乙酰胆碱(Ach)的作用。方法:①采用哮喘造模豚鼠分为中药敷贴组、模型对照组、氨茶碱注射组、氨茶碱敷贴组,治疗1周后记录哮喘潜伏时间;②体外实验中,增加1组空白对照组,观察血清直接对抗由Ach引起豚鼠体外螺旋气管条的收缩,计算收缩百分比;③采用同浓度的药物血清对抗组胺(His)。结果:中药循经敷贴法能对抗豚鼠的致敏哮喘,延长哮喘潜伏时间;具有显著对抗Ach作用,但同浓度药物血清无对抗His作用。结论:对抗Ach解痉作用是该中药循经敷贴法治疗哮喘的重要机制之一。

Discussion of mechanism of herbal penetration treatment along channel for asthma

Objective: To observe the effect of herbal penetration treatment along channel for the latent period of asthmatic attack in guinea pig and the affection of herbal containing serum against acetylcholine(Ach).Methods:① Guinea pigs of asthma models were divided into 4 groups,treatment group with herbal penetration along channel,control group,aminophyline injection group and aminophyline penetration group.After one week of treatment,the latent period of asthmatic attack was recorded.②In vitro experiment,one blank group was added to observe the direct action of serum against the spiral tracheal strip constriction induced by Ach.The relaxation rate was calculated.③The same concentration of serum was used to observe the action against histamnias induced constriction.Results: Herbal penetration treatment could inhibit sensitized asthma,extend the latent period of asthmatic attack.Herbal containing serum has obvious anti-acetylcholine function,however the same concentration of serum does not affect on antihistamic.Conclusion: Anti-acetylcholine and relax tracheal constriction may be one of the mechanisms of herbal penetration treatment along channel for asthma.