一测多评法同时测定野菊花中7种成分

目的建立野菊花Chrysanthemum indicum中绿原酸、咖啡酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸、蒙花苷、木犀草素、芹菜素7种成分一测多评测定方法。方法采用HPLC法,以绿原酸为内参物,建立绿原酸与咖啡酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸、蒙花苷、木犀草素、芹菜素的相对校正因子,并考察了相对校正因子的重现性,分别采用外标法和一测多评法进行野菊花中7种成分的测定。结果建立的校正因子重现性良好,野菊花药材采用一测多评法计算的量值与外标法实测值之间没有明显差异。结论同时测定7种成分的一测多评法控制野菊花药材的质量是可行、准确的。

Simultaneous determination of seven components in Chrysanthemum indicum by QAMS

Objective To establish a new method for the quantitative analysis of multi-components by single-marker (QAMS) to simultaneous determine seven components in Chrysanthemum indicum. Methods The chlorogenic acid was used as internal marker to calculate the relative correlation factors (RCF) of caffeic acid, luteolin-7-O-glucoside, 3,5-O-dicaffeoylquinic acid, buddleoside, luteolin, and apigenin by high performance liquid chromatography (HPLC). The repeatability of RCF was investigated. The contents of seven components were determined by the external standard method and QAMS respectively. Results The reproducibility of RCF was perfect. The value calculated by QAMS was consistent with the external standard method. Conclusion The QAMS method for simultaneously measuring the content of seven components is feasible and accurate to evaluate the quality of Chrysanthemum indicum.