胃癌p53表达及DNA倍体研究

本文应用ＬＳＡＢ法对７５例胃癌及癌旁组织进行了ｐ５３表达的研究，并分析了ｐ５３表达与胃癌ＤＮＡ倍体的关系。结果：７５例胃癌中ｐ５３表达阳性率为４１．３％。４例癌旁重度异型增生中有２例表达阳性，ｐ５３在早期胃癌中阳性率（６０．０％）高于进展期胃癌（３８．５％）；肠型胃癌中阳性率（５２．６％）明显高于弥漫型胃癌（２９．７％）（Ｐ＜０．０５）；异倍体胃癌ｐ５３阳性率（５１．１％）明显高于二倍体胃癌（２６．７％）（ｐ＜０．０５）。经统计分析，未见ｐ５３表达与胃癌的恶性程度指标之间有明显关系。结果说明，ｐ５３表达与胃粘膜癌变的早期阶段关系密切，主要与肠型胃癌和异倍体胃癌发生有关。

EXPRESSION OF p53 AND DNaA PLOIDY IN GASTRIC CANCER

y means of LSAB technicque and lmage Cytometry analysis,expression of p53 andDNA ploicy were examined in 75 cases of gastric cancer(GC).The p53 positive expressionwas found in 31 of 75 cases(41.5%),and in severe dysplasia lesions adjacent to carcinomtous sites in 2 of 4 cases,The positive rate of p53 in early cases was 60.0%,higher thanthat in advanced ones(38.5%).The positive rate of p53 in the intestinal type carcinomas(52.6)was significantly higher than that in the diffuse type(29.7%)(P<0.05);The obser-vation of Image Cytometry analysis showed 51.1%positive rate of p53 in aneuploidy and on-ly 26.7%in diploidy(P<0.05).No association was found between expression of p53 anddegree of GC malignancy. Therefore,p53 mutation mainly plays a role in intestinal type andaneuploidy pattern carcinomas. p53 abnormality was the early sign of gastric carcinogenesis. It indicated that the examination of p53 is helpful in the diagnosis of early GC.