三磷酸腺苷对骨髓间充质干细胞促进脊髓损伤后肢体功能恢复的影响

目的观察三磷酸腺苷（ATP）对骨髓间充质干细胞（BMSCs）促进脊髓损伤（SCI）后肢体功能恢复的影响。方法将48只体重为240～260 g的SD雌性大鼠,采用改良Allens打击法制备T₁₂节段SCI模型后,随机分为4组,每组12只,A组（ATP+BMSCs联合移植组）、B组（BMSCs移植组）、C组（ATP移植组）、D组（空白对照组）。术后观察大鼠一般情况,于1、3、7、14、21、28 d采用Tarlov评分评价后肢功能恢复程度;28 d处死大鼠后取材进行SP染色观察SCI修复情况。结果术后各组大鼠均出现后肢瘫痪症状,2～3周开始有不同程度恢复,以A组为最快,B、C组次之,D组最差。术后14、21、28 d各组改良Tarlov评分除B、C组比较差异无统计学意义外,其他各组比较差异均有统计学意义（P<0.05）。术后28 d SP染色观察显示,A、B组可见存活的BrdU标记阳性BMSCs,A组BrdU阳性细胞数多于B组,差异有统计学意义（P<0.05）;C、D组未见BrdU标记阳性细胞。结论 ATP对BMSCs移植修复SCI后肢体功能的恢复具有协同促进作用。

Effect of adenosine triphosphate on the functional recovery of rats spinal cord injury after transplantation of bone marrow-mesenchymal stem cells

Objective To study the effect of adenosine triphosphate（ATP） in aiding the functional recovery in rats spinal cord injury（SCI） after transplantation of bone marrow-mesenchymal stem cells（BMSCs） delivery on lesion site.Methods 48 experimental adult female Sprague dawley rats（weighing 240～260g） were made SCI models at T₁₂ levels according to the improved Allen’s method and were divided into 4 groups randomly.Group A,ATP（40 mg/kg） and BMSCs（6μL） were injected to the SCI;group B,only BMSCs were injected;group C,only ATP was injected; group D only laminectomy.The general conditions and their motility of hind limb were regularly scored using the improved Tarlov scale on 1,3,7,14,21 and 28 days after operation.After 4 weeks,rats were sacrificed,and their spinal cords were examined for 5-bromo-2-deoxy uridine labeled BMSCs by immunostainings.Results Paralysis symptom in hind limb occurred in all experimental rats.There was gradual improvement noticed in group A,B and C after the operation and significant motor recovery in group A at 2～3 weeks.No obvious change was observed in group D.The scores,except those between groups B and C,showed significant differences among groups on 14,21 and 28 days after operation（P <0.05）.The ultrastructures of spinal cord tissue showed immunohistochemical positive staining cells with antibody of anti-5-bromo-2-deoxy uridine in groups A and B on 28 days after operation,and the cells of group A was significantly more than those of group B（P <0.05）.There were no positive staining cells in groups C and D. Conclusion ATP had the effect of synergistical action on reparation using BMSCs in SCI.