人类白细胞分化抗原133与乳腺浸润性导管癌临床病理特征的关系

目的 研究肿瘤干细胞标志物人类白细胞分化抗原（CD）133在乳腺浸润性导管癌组织中的表达及其与临床病理因素之间的关系.方法 收集2001年1月至2005年12月间承德市肿瘤医院和承德医学院附属医院肿瘤科的102例乳腺浸润性导管癌组织,采用免疫组织化学方法检测其CD133蛋白表达,并用χ^2验或Fisher确切概率法分析CD133表达与临床病理因素的关系.结果 乳腺浸润性导管癌组织不同程度地表达CD133.不同肿瘤直径组间CD133阳性表达率差异有统计学意义（χ^2=7.476,P=0.024）,其中肿瘤直径〉2～5 cm组CD133阳性表达率明显高于肿瘤直径≤2 cm组56.72%（38/67）比26.09%（6/23）,χ^26.429,P＆lt;0.012].不同组织学分级间相比,CD133阳性表达率的差异也有统计学意义（χ^26.274,P=0.043）.并且,有淋巴结转移者CD133阳性表达率比无淋巴转移者高64.71%（22/34）比39.71%（27/68）,χ^25.675,P=0.017）].乳腺浸润性导管癌组织中,CD133阳性表达率在不同年龄、不同临床分期之间差异无统计学意义（χ^20.177,P=0.674;χ^22.874,P=0.242）.结论 CD133有可能作为判断乳腺浸润性导管癌侵袭转移的指标.

Relationship between CD133 expression and the clinicopathological characteristics of breast invasive ductal carcinoma

Objective To investigate the expression of cancer stem cell marker CD133 in breast invasive ductal carcinoma and its relationship with the clinicopathological parameters. Methods Totally 102 cases of invasive ductal carcinoma in the Tumour Hospital of Chengde City and Department of Oncology, the Affiliated Hospital of Chengde Medical College from January 2001 to December 2005 were involved, and CD133 protein expressions in their carcinoma tissues were detected by immunohistochemistry. χ^2 test or Fisher probabilities test was used to analyze the relationship of CD133 expression and elinicopathologieal parameters. Results CD133 was expressed to different extents in the breast invasive ductal carcinoma tissues. The positive expression rate of CD133 showed statistical significant difference between groups with different tumor sizes （ χ^2 = 7. 476,P=0. 024） ,and the positive expression rate of CD133 in the patients with tumor diameter of 〉2-5 cm was significantly higher than that in the patients with tumor diameter ≤ 2 cm 56. 72% （38/67）vs 26. 09% （ 6/23 ） , χ^2 = 6. 429, P〈0. 012 ]. There were some statistical differences between groups at different histological grades（X^2 =6. 274,P=0. 043）. The positive expression rates of CD133 in patients with lymph node metastasis were significantly higher than those without 64. 71% （22/34） vs 39. 71% （27/68） ,χ^2 =5. 675 ,P=O. 017 ~. There was no significant difference in the expression of CD133 between groups with different age and dinieal stage （ X^2 = 0. 177,P = 0. 674 ;X^2 = 2. 874, P = 0. 242 ）. Conclusion CD133 can be used as an important markerof invasion and metastasis in breast invasive ductal carcinoma.