血清IL-25与HIV合并机会性感染的相关性

目的：探讨人类免疫缺陷病毒（HIV）感染者血清IL-25水平与机会性感染的关系。方法收集55例HIV感染者的临床资料，并应用ELISA法检测血清IL-25浓度，流式细胞术检测CD4＋ T细胞计数，分析两者与HIV感染者发生机会性感染的相关性。结果55例患者中有44例（80.00%）发生机会性致病菌感染，其中26例（47.27%）发生口腔念珠菌感染。发生机会性感染组患者血清IL-25水平较未发生感染组高（946.45±1652.84）pg/ml vs （107.58±167.33）pg/ml，P ＜0.01），CD4＋ T细胞计数降低（87.54±110.60）cell/μl vs （298.60±211.91）cell/μl，P ＜0.05）。发生口腔念珠菌感染组较未发生感染组患者血清IL-25增高（1247.78±1877.48）pg/ml vs （358.09±944.31）pg/ml，P＜0.05），CD4＋ T细胞计数降低（48.56±74.90）cell/μl vs （200.04±182.79）cell/μl，P ＜0.05）。相关性分析显示，IL-25浓度、CD4＋ T细胞计数分别与发生机会性感染、口腔白色念珠菌感染呈正相关、负相关（P ＜0.01）。结论 IL-25可能参与HIV感染者发生机会性感染后的免疫反应，高IL-25水平可能与HIV感染者易患机会性感染、口腔念珠菌感染有关。

Relationships between serum concentration of interleukin-25 and opportunistic infections in patients with HIV infection

Objective To investigate the relationships of serum concentration of interleukin-25 （IL-25） and opportunistic infections of patients with HIV infection. Methods The serum samples and the clinical information of 55 cases with HIV infection were collected. The serum concentrations of IL-25 and CD4＋ T-cell count were measured by enzyme linked immunosorbent assay （ELISA） and flow cytometry, respectively. Results There were 44 cases （80.00%） suffering with opportunistic infections among 55 cases and 26 cases （47.27%） were infected with oral candidiasis. Higher serum level of IL-25 （946.45 ± 1652.84） pg/ml vs （107.58 ± 167.33） pg/ml, P 〈 0.01] and lower CD4＋ T-cell count （48.56 ± 74.90 cell/μl vs （200.04 ± 182.79） cell/μl, P 〈 0.01] were found in patients with opportunistic infections than the cases without opportunistic infections. The serum IL-25 was higher in cases with oral candidiasis than the others （1247.78 ± 1877.48） pg/ml vs （358.09 ± 944.31） pg/ml, P 〈 0.05], while the CD4＋ T-cell count was significantly lower in patients with oral candidiasis than that in patients without candidiasis （48.56 ± 74.90） cell/μl vs （200.04 ± 182.79） cell/μl, P 〈 0.01]. Serum IL-25 and CD4＋ T-cell count were positive and negative correlated with opportunistic infection and oral candidiasis, respectively. Conclusions IL-25 may play a role in the immunologic mechanism of HIV patients with opportunistic infections. Higher serum IL-25 is probably related to high susceptibility of opportunistic infection and oral candidiasis.