新疆维吾尔族HLA—A、HLA-B基因多态性与HBV感染结局的相关性

目的：研究HLA-A、HLA-B基因多态性与新疆维吾尔族人群HBV感染结局的相关性。方法临床收集自限HBV感染者50例（RHBS组）、慢性HBV携带者80例（ASC组）、CHB患者100例（CHB组），采用PCR-SSP法检测HLA-A、HLA-B基因型，比较组间基因频率差异。结果 HLA-A＊33在CHB组分布频率（2.50%）显著低于ASC组（8.75%），差异具有统计学意义（χ2=7.355，P =0.007，OR =0.248，95%CI 0.085～0.722）。HLA-A＊33在RHBS组分布频率（1.00%）低于ASC组（8.75%）（χ2=7.242，P =0.007，OR =0.96，95%CI 0.012～0.756），差异具有统计学意义。HLA-B＊52在CHB组分布频率（7.50%）高于ASC组（1.88%），差异具有统计学意义（χ2=8.757，P =0.003，OR =5.634，95%CI 1.596～19.887）。HLA-B＊52在RHBS组分布频率（11.00%）高于ASC组（1.88%）（χ2=10.665，P =0.001，OR =7.239，95%CI 1.908～27.467），差异具有统计学意义。结论 HLA-A、HLA-B基因多态性影响HBV感染临床结局，HLA-A＊33基因与病毒携带状态有关。HLA-B＊52基因具有较强的抗HBV感染能力，临床上易表现为HBV一过性感染或慢性乙型肝炎。

Association of HLA-A,HLA-B polymorphism with the outcomes of hepatitis B virus infection in Uygurpopulation of Xinjiang

Objective To invest the associations of human leukocyte antigen （HLA）-A, HLA-B polymorphism with the outcome of HBV infection and the replication of HBV in Uygur population of Xinjiang. Methods Total of 50 patients with resolved from HBV infection, 80 asymptomatic HBV carrier patients and 100 chronic hepatitis B patients were included in our study. HLA-A, HLA-B genotyping was conducted with PCR-SSP. The frequency distributions of genotype were analyzed. Results The frequency of HLA-A＊33 allele distribution in CHB group （2.50%） was significantly lower than ASC group （8.75%, χ 2 =7.355, P = 0.007, OR = 0.248, 95%CI 0.085-0.722）. The frequency of HLA-A＊33 allele distribution in RHBS group （1.00%） was significantly lower than ASC group （8.75%, χ 2 = 7.242, P = 0.007, OR = 9.750, 95%CI 2.038-46.645）. The frequency of HLA-B＊52 allele distribution in CHB group （7.50%）was significantly higher than ASC group （1.88%, χ2 = 8.757, P = 0.003, OR = 5.634, 95%CI 1.596-19.887）. The frequency of HLA-B＊52 allele distribution in RHBS group （11.00%） was significantly higher than ASC group （1.88%, χ 2= 10.665, P = 0.001, OR = 7.239, 95%CI 1.908-27.467）. Conclusions HLA-A, HLA-B gene polymorphism may play an important role in determining the outcomes of hepatitis B virus infection in Chinese Uygur population of Xinjiang. The HLA-A＊33 allele could aggravate persistant infection of HBV. HLA-B＊52 allele could keep individuals away from HBV infection and was closely related with the outcomes of resolving from HBV infection spontaneously and CHB.