p53,p16,PCNA蛋白在食管癌中的表达及其临床意义

目的探讨p53,p16,PCNA蛋白在食管癌中的表达及临床意义。方法用免疫组织化学染色SP法对62例食管癌标本进行p53,p16,PCNA蛋白测定。结果62例食管癌中,p53,PCNA蛋白阳性表达均为71.0%(44/62),p16缺失率48.4%(30/62)。p16缺失与肿瘤浸润深度、淋巴结转移密切相关(P<0.05、P<0.01)。而p53,PCNA蛋白同时表达56.5%(35/62),亦与肿瘤浸润深度、淋巴结转移密切相关(P<0.05、P<0.01)。结论食管癌p53,PCNA蛋白同时表达及p16缺失可视为危险预后因素。

Clinical Value of the Expression of p53, p16 and PCNA Protein in Esophageal Carcinoma

Objective To investigate the expression of p53, p16 and proliferating cell nuclear antigen (PCNA) protein in esophageal carcinoma and its clinic significance. Methods The expression of p53, p16 and PCNA protein in 62 cases with esophageal carcinoma were detected by immunohistochemical assay (S-P technique). Results The positive expression rate of p53 or PCNA protein in the 62 cases was equal to 71.0% (44/62). The rate of p16 loss was 48.4% (30/62).The loss of p16 was significantly related to the invasive depth and the lymph node metastasis (P<0.05, P<0.01). The positive expression of p53 or PCNA (56.5%, 35/62) was significantly related to the depth of invasion and lymph node metastasis, too (P<0.05, P<0.01). Conclusion The expressions of p53 or PCNA and the loss of p16 in esophageal carcinoma may serve as risk prognostic factors.