自体施万细胞神经膜管移植修复大鼠脊髓损伤的实验研究

目的探讨自体施万细胞神经膜管（SCNC）移植对脊髓半横断损伤的修复作用。方法成鼠30只，随机分3组：1O只造模后行SCNC移植（A组）；10只造模但不移植（B组）；10只不造模也不移植作正常对照组（C组）。下段胸髓半切法造模。术后8周每周行动物行为测试和斜板试验；术后6周测定皮层体感诱发电位（CSEP）；术后8周行组织学观察及计算机图像分析。结果A组、C组可测出CSEP波形，B组未测出。行为测试、斜板试验及图像分析A组、C组与B组差异显著，A组与C组之间斜板试验有显著差异。A组通过脊髓损伤处的再生神经纤维数量明显多于B组。结论自体施万细胞神经膜管移植有助于引导再生轴突生长通过，对损伤脊髓再生有较明显的促进作用。

Repairing of Spinal Cord Injury with Schwann Cell Neurilemma Channel in Rats

ObjectiveTo apply the Schwann cell neurilemma channel(SCNC) in promoting the regeneration of injuried spinal cord.MethodsSpinal cords of rats after laminectomy at T8/9 were divided in 3 groups randomly: 10 rats were transplanted with SCNC(Group A);10 rats only made models(Group B);10 rats were as normal control group(Group C).All rats received behaviour survey and inclined plane test every week 8 weeks after operation.6 weeks after operation,they were measured with cortical somatosensory evoked potential(CSEP).And 8 weeks after operation,all specimens were studied histologically.ResultsCSEP could be recorded in group A and C,but not in group B.There were marked differenties between group A or C and group B in behavior survey,inclined plane test.Amount of nerve fibers regenerated through the injury gap in group A was significantly larger than that in group B.ConclusionSCNC could facilitate axonal regeneration and promote the repairing of injuried spinal cord.