| Wortmannin对表皮生长因子诱导的血管平滑肌细胞增殖及迁移的影响 |
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| 引用本文： | 陈龙菊,卢进昌,姜铧,梁江红,刘志祥,张正洪,王汉琴. Wortmannin对表皮生长因子诱导的血管平滑肌细胞增殖及迁移的影响[J]. 郧阳医学院学报, 2008, 27(5) |
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| 作者姓名： | 陈龙菊 卢进昌 姜铧 梁江红 刘志祥 张正洪 王汉琴 |
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| 作者单位： | [1]郧阳医学院解剖学教研室,湖北十堰442000 [2]郧阳医学院附属太和医院生命科学研究所,湖北十堰442000 [3]郧阳医学院附属太和医院呼吸科,湖北十堰442000 [4]郧阳医学院附属太和医院血液科,湖北十堰442000 [5]郧阳医学院附属太和医院妇产科,湖北十堰442000 |
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| 摘 要： | 目的:研究PI3K抑制剂Wortmannin对表皮生长因子(EGF)诱导的大鼠血管平滑肌细胞(VSMCs)增殖、迁移及磷酸化Akt蛋白表达的影响,旨在探讨EGF诱导的VSMCs增殖和迁移的信号通路。方法:以低血清培养的VSMCs作为对照,采用细胞计数、划痕损伤实验和WesternBlotting技术,观察Wortmannin对EGF诱导的VSMCs增殖、迁移及磷酸化Akt蛋白表达的影响。结果:干预后24h,EGF组VSMCs增殖、迁移较对照组明显增强,同时磷酸化Akt蛋白的表达平行增高。而Wortmannin则明显抑制了VSMCs的增殖、迁移和磷酸化Akt蛋白的表达。结论:PI3K/Akt信号通路参与了EGF诱导的VSMCs增殖和迁移。
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| 关 键 词： | Wortmannin 血管平滑肌细胞 增殖 迁移 表皮生长因子 |
Effects of Wortmannin on EGF-Induced Proliferation and Migration of Vascular Smooth Muscle Cells |
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| CHEN Long-ju,,LU Jin-chang,JIANG Hua,LIANG Jiang-hong,LIU Zhi-xiang,ZHANG Zheng-hong,WANG Han-qin. Effects of Wortmannin on EGF-Induced Proliferation and Migration of Vascular Smooth Muscle Cells[J]. Journal of Yunyang Medical College, 2008, 27(5) |
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| Authors: | CHEN Long-ju LU Jin-chang JIANG Hua LIANG Jiang-hong LIU Zhi-xiang ZHANG Zheng-hong WANG Han-qin |
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| Affiliation: | CHEN Long-ju1,2,LU Jin-chang3,JIANG Hua4,LIANG Jiang-hong5,LIU Zhi-xiang2,ZHANG Zheng-hong1,WANG Han-qin2(1 Department of Anatomy,Yunyang Medical College,2 Institute of Life Scince,3 Department of Respiration,4 Department of Blood,5 Department of Gynaecology , Obstetrics,Taihe Hospital,Shiyan,Hubei 442000,China) |
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| Abstract: | Objective To study the effects of Wortmannin,inhibitor of PI3K on proliferation and migration of vascular smooth muscle cells induced with epidermal growth factor(EGF)and the expression of Akt protein.Methods The rat VSMCs cultured in low serum medium was served as a control group.The proliferation and migration of VSMCs and the expression of Akt protein was measured by cell counting,scratch wound experiment and Western blotting analysis,respectively.Results The proliferation and migration of VSMCs in EGF-i... |
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| Keywords: | Wortmannin |
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