Stimulatory effect of ionophores on adenosine 3',5'-monophosphate content in human mononuclear leukocytes |
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Authors: | V Stolc |
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Institution: | Department of Pathology, School of Medicine, University of Pittsburgh. Pittsburgh, PA 15261, U.S.A. |
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Abstract: | Ionophores A23187 and bromo-lasalocid ethanolate enhanced the cyclic AMP content in human mononuclear leukocytes. The maximum effect of A23187 with a 10-min incubation was found with 0.3–1.0μM concentrations with or without l-isoproterenol (1 μM) or prostaglandin E 1 (pge 1) (0.3 μM). The maximum effect after 5 min of incubation at 37° was observed with 0.05, 0.2 and 1 μm A23187. The effect of ionophore A23187 was enhanced by both aminophylline (1 mM) and isobutyl-methylxanthine (1 mM). Calcium (1 mM). aspirin (1 mM) and indomethacin (100 μM) decreased the stimulatory action of A23187. Bromo-lasalocid ethanolate increased cyclic AMP content in cells maximally at a 3 μM concentration with or without 0.3 μM pge 1. |
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Keywords: | cyclic AMP adenosine 3' 5'-monophosphate |
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