| 光学及荧光显微镜在细胞凋亡检测中的应用 |
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| 引用本文： | 熊琛,郭怀兰,陈绪云,杨靖.光学及荧光显微镜在细胞凋亡检测中的应用[J].华中科技大学学报(医学版),1998(3). |
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| 作者姓名： | 熊琛 郭怀兰 陈绪云 杨靖 |
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| 作者单位： | 十堰市人民医院医学病毒学研究所 十堰442000(熊琛)，郧阳医学院基础部预防医学教研室 十堰442000(郭怀兰)，郧阳医学院基础部微生物学教研室 十堰442000(陈绪云，杨靖) |
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| 摘 要： | 为观察细胞培养中细胞凋亡的形态及数量,取4℃环境下不同时间培养的小鼠胸腺细胞,用光镜或吖啶橙(AO),溴化乙锭(EB)染色后荧光镜观察。光镜下可见凋亡小体,荧光镜下早期凋亡细胞发绿色或暗绿色荧光,伴有核结构的变化,而晚期凋亡细胞发橙红色荧光并有凋亡小体的形成。结果表明:光镜下需出现典型核形态变化时凋亡才能确认,AO和EB双重荧光染色后荧光镜检在细胞凋亡检测中既可定性,又可定量。
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| 关 键 词： | 凋亡 染剂和染色法 细胞 培养的 |
Application of Light Microscope and Fluoroscope in Detection of Apoptosis |
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| Xiong Chen,Guo Huailan,Chen Xuyun et al Institute of Medical Virology,Shiyan People's Hospital,Shiyan.Application of Light Microscope and Fluoroscope in Detection of Apoptosis[J].Journal of Huazhong University of Science and Technology(Health Sciences),1998(3). |
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| Authors: | Xiong Chen Guo Huailan Chen Xuyun Institute of Medical Virology Shiyan People's Hospital Shiyan |
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| Institution: | Xiong Chen1,Guo Huailan2,Chen Xuyun3 et al1 Institute of Medical Virology,Shiyan People's Hospital,Shiyan 4420002 Department of Preventive Medicine,3 Department oj Microbiology,Yunyang Medical College,Shiyan 442000 |
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| Abstract: | To observe the morphology and amount of apoptosis cells, the authors detected murine thy-mocytes prepared at 4 C with different lengths of time by light microscope or fluoroscope after AO EB staining. Under light microscope apoptosis bodies were found, while under fluoroscope early apoptosis cells accompanied with nuclear morphologic changes emitting green or dark green fluorescence and late apoptosis cells scintillating reddish orange fluorescence often had apoptosis bodies. The results showed that apoptosis was identified only when the typical change of nuclear morphology appeared under light microscope and that both qualitative and quantitative analysis for determining apoptosis could be done by the double fluorescence staining technique. |
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| Keywords: | apoptosis stains and staining cells cultured |
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