The cellular transfer of immunity to Trichostrongylus colubriformis in an isogenic strain of guinea-pig: III. The localization and functional activity of immune lymph node cells following syngeneic and allogeneic transfer

Mesenteric lymph node cells obtained from guinea-pigs immunized with Trichostrongylus colubriformis have been transferred allogeneically (McMaster outbred to Heston inbred) and syngeneically (Heston to Heston inbred) by intravenous injection. The immune cells were transferred on days 6, 7 and 8 of infection when the parasite was in the susceptible fourth stage of development. The syngeneically transferred cells caused rejection of the parasite but the allogeneically transferred cells were not effective.

Immune mesenteric lymph node cells were labelled in vitro with ⁵¹Cr before transfer to infected recipients. The percentage of the intravenously injected cells which localized in infected small intestine has been calculated from γ-counts recorded at 1, 6, 16, 24 and 48 hours after injection of the labelled cells. The mean percentage of cell dose per 12 in. of small intestine recorded following syngeneic transfer was 1·29 and following allogeneic transfer 0·68. A significant difference in the localization of allogeneic and syngeneic cells in the small intestine was already apparent at 6 hours following intravenous injection.