不同几丁糖-胶原膜物理性能的比较研究

目的以几丁糖和胶原为主要材料制作可吸收膜并对膜的物理性能进行比较。方法从牛肌腱中提取胶原。将2％几丁糖乙酸溶液风干成膜，在其上流延0．1％胶原乙酸水溶液，风干后制成复合膜；不同量的胶原乙酸水溶液与2％几丁糖乙酸溶液混合，风干后为混合膜；将混合液冷冻干燥制成冻干膜。测定膜表面结构和成分、吸水性及抗张强度。结果复合膜质地不均而混合膜和冻干膜质地均匀。随着胶原量及膜厚度的增加，膜均匀性更加。复合膜表面不平整、无孔隙、有胶原聚集，而混合膜和冻干膜表面均匀呈多孔状。与几丁糖膜比较，复合膜的红外透射光谱与几丁糖膜基本一致，但混合膜差异较大。冻干膜的吸水性较其他两种膜高。同样比例的复合膜与混合膜抗张强度无明显差异，但均大于冻干膜；随着胶原量的增加膜的强度增加。结论几丁糖与胶原有良好的成膜性。混合膜和冻干膜适用于引导骨再生膜。

Comparison of mechanical properties of different chitosan-collagen absorbable membranes

Objective To prepare absorbable membranes with collagen and chitosans in different ways, and to compare their physical properties. Methods Collagen was extracting from bovine tendon. 2% chitosan acetic acid solution was air-dried to form the membrane, which was covered by 0.1% collagen solution, the composite membrane was air-dried. Collagen acetic acid solution was mixed with chitosan acetic acid solution, and air-dried to form compound membranes. Frozen-dried membrane was acquired from mixed solution of collagen and chitosan by freezing and desiccation. Membrane surface structure was observed by scan electron microscopy and water absorbent property and tensile strength were assessed. Infrared spectral analysis was used to analyze membrane ingredients. Results The texture of the chitosan- collagen composite membrane was uneven, while the compound membrane and frozen-dried membrane had uniform texture. In addition, with collagen content and thickness increasing, the texture becomes more uniform. The composite membrane was less smooth on its surface than compound membrane with congregations of collagen fibers and pore-free. However, compound membrane and frozen-dried membrane were porous on the surface. Comparing with chitosan membrane, the infrared transmitted spectrum was almost the same as composite membrane, while compound membrane was significant difference. Frozen-dried membrane had significantly higher water absorbent property than the other two. The tensile strength was similar between compound and composite membranes, both were significantly higher than that of frozen-dried membrane. With the increasing of collagen, the tensile strength enhanced which was as same as water absorbent property. Conclusion Chitosan and collagen have good membrane forming property. Both compound and frozen-dried membrane seem to be suitable for guiding bone regeneration membrane.