反复吗啡处理对小鼠淋巴细胞Ca~（2＋）含量膜流动性及原癌基因表达的影响

应用递增剂量急性吗啡处理４天后，小鼠胸腺细胞或脾细胞［Ｃａ２＋］ｉ下降，脾淋巴细胞膜脂流动性降低，同时ＣｏｎＡ活化的脾脏Ｔ细胞表达Ｃ－ｍｙｂ和Ｃ－ｍｙｃｍＲＮＡ明显减少，提示反复吗啡处置对免疫细胞内的信息传导系统及膜脂系统已产生明显影响。

EFFECTS OF REPITITVE IN VIVO TREATMENTS OF MORPHINE ON LYMPHOCYTE INTERPLASMIC CONTENT OF Ca2+,MEMBRAME FLUIDITY AND PROTO-ONCOGENES EXPRESSION

ymphocyte activation is closely interrelated with transmembrane Ca2+influx, proto-oncogenes induction and membrane lipid fluidity.The microfluorometric measurement shows that the cytoplasmic free Ca2+ in both thymo-cytes and splenocytes of Mor-treated mice decreased obviously,as compared with the control. The determination of plasma membrane fluidity by fluorescence polarization technique reveals that splenocytes of Mor-treated mice possessed a much reduced membrane lipid fluidity.Northern blot hybridization results show unfractionated spleno-cytes were polyclonally stimulated by con A to express C-myb,C-myc-specific mRNA with detectable levels 6 hrs thereafter,and to remain high level at 48hrs post stimulation. Quantitation by densitometric scanning indicated both C-myb and C-myc mRNA expression in splenocytes of Mor-treated mice were detected to be significantly de-creased at the aforementioned two time points .In sumrnary,repititive in vivo treatments of Mor produced damages in both cell signal transduction and membrane lipid fluidity in the immune system-