磷酸化AKT与磷酸化EGFR在非小细胞肺癌中的表达及意义

目的:评价磷酸化表皮生长因子受体(phosphorylated epidermal growth factor receptor,p-EGFR)及其信号转导通路下游分子AKT的活化形式磷酸化AKT(phosphorylated AKT,p-AKT)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其意义.方法:免疫组织化学法检测95例NSCLC组织中p-AKT和p-EGFR蛋白的表达,并分析2种蛋白表达之间的关系及与预后的相关性.结果: p-EGFR的阳性表达率为57.89%(55/95),在低年龄组(33/46)、女性组(24/30)和腺癌组(35/52)的表达率较高,差异有统计学意义(P=0.008, P=0.003, P=0.009).p-AKT的阳性表达率为66.32%(63/95),在低分化组(20/23)、有淋巴结转移组(29/37)和肿瘤较小(≤3 cm)组(36/46)的表达率较高,差异有统计学意义(P=0.036,P=0.047,P=0.017).单因素分析显示,p-AKT和p-EGFR蛋白表达与预后无关(P=0.854,P=0.729).多因素分析显示,2种蛋白表达与生存时间也无关(P=0.497,P=0.731).p-AKT表达与p-EGFR表达呈正相关性(r=0.204,P=0.047).结论:p-EGFR和p-AKT的阳性表达率对NSCLC患者预后无提示意义,二者均不能成为预后指标.

Expressions of phosphorylated AKT and phosphorylated EGFR in non-small cell lung cancer and their significance

Objective:To evaluate the expressions of phosphorylated epidermal growth factor receptor (p-EGFR) and its downstream molecule phosphorylated AKT(p-AKT) in non-small cell lung cancer (NSCLC)and their significance. Methods:The immunhistochemistry was used to detect the expressions of p-AKT and p-EGFR proteins in 95 cases of NSCLC tissues. The relationship between the p-AKT and p-EGFR and between their expression and prognosis were also evaluated. Results:The positive rate of p-EGFR was 57.89% (55/95) and was related with age, gender and histological type (P=0.008,P=0.003,P=0.009). The positivity of p-EGFR was higher in young group (33/46), female group (24/30), and adenocarcinoma group (35/52). The positive rate of p-AKT was 66.32% (63/95) and was related with differentiation, lymph node metastasis and tumor size (P=0.036,P=0.047,P=0.017,res-pectively). The positivity of p-AKT was higher in low differentiation group (20/23), lymph node metastasis group (29/37), and tumor diameter ≤3 cm group (36/46). Univariate analysis showed that the expressions of p-AKT and p-EGFR were not associated with prognosis (P=0.854, P=0.729). Multivariate analysis showed that neither p-AKT nor p-EGFR was correlated with the patients' survival time (P=0.497, P=0.731). The p-AKT was positively correlated to the p-EGFR(r=0.204, P=0.047). Conclusion:The expressions of p-AKT and p-EGFR had no impact on the prognosis of NSCLC. They can not serve as prognostic indicators for NSCLC.