Muscle injury induced by different types of contractions in dystrophic mdx mice |
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Authors: | Lou Jianwei Bi Wenbo Li Wei Zhao Yuying Liu Shuping Zheng Jinfan Yan Chuanzhu |
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Institution: | (1) Laboratory of Neuromuscular Disorders and Department of Neurology, Qilu Hospital, Shandong University, 107 West Wenhua Road, Jinan, 250012, People’s Republic of China;(2) School of Mechanical Engineering, Shandong University, 17923 Jingshi Road, Jinan, People’s Republic of China; |
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Abstract: | Studies on comparing the effect of lengthening, isometric and shortening contractions on dystrophin-deficient muscles are
unavailable. We hypothesized that different types of contractions lead to different extents to which dystrophin-deficient
muscles are injured. For this purpose, we developed protocols for different types of contraction-induced injury to mdx muscles in vitro. Force deficits and percentages of procion orange dye positive fibers were employed to assess the extent
of injury to each muscle. Our results revealed that both the lengthening and isometric contractions resulted in significantly
greater injury to extensor digitorum longus (EDL) muscles of mdx mice than to that of control (C57BL/6) mice. In contrast, the shortening contractions induced very mild and identical injury
to EDL muscles of mdx and C57BL/6 mice. Then another protocol was carried out in vivo to ascertain the effect of shortening contractions on mdx
muscles by achillotenotomy. Histological assessment revealed that the triceps surae muscles with excised Achilles tendon (EAT)
displayed little and significantly milder injury than the normal ones did. In conclusions, the unloaded shortening contractions
induce little injury to mdx muscles. The in vitro protocol for different types of contraction-induced injury is sensitive
and reliable. |
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