瘦素及瘦素受体基因多态性与乳腺癌的相关性研究

目的探讨瘦素及其瘦素受体基因多态性与乳腺癌发生的关系。方法采用PCR- RFLP对94例乳腺癌患者、128例健康对照者进行瘦素受体基因Gln223Arg多态性检测;ELISA分析法测定瘦素水平。结果乳腺癌组瘦素受体基因Gln223Arg的GG、GA和AA基因型表达频率分别为69.15%、17.02%和13.83%;等位基因G和A为77.66%和22.34%与对照组82.03%、15.63%和2.34%及等位基因的89.84%和10.16%相比较,差异有统计学意义(P=0.004,P=0.001)。乳腺癌组瘦素水平,腰臀比(WHR)明显高于对照组,差异均有统计学意义(P<0.01,P<0.001)。非条件logislic回归多因素分析表明,瘦素受体基因多态性、瘦素水平及WHR升高,与乳腺癌发生的相关危险度分别为:OR=4.87,95%CI:1.30-18.22,P=0.019;OR=1.53,95%CI:1.13-2.07,P= 0.006;OR=3.68,95%CI:1.34-10.11,P=0.011。结论瘦素受体基因Gln223Arg多态性、瘦素及WHR升高,可能增加乳腺癌发生的风险性。

Association among Iipids, 1eptin and leptin receptor polymorphisms with risk of breast cancer

Objective To evaluate the association between serum level of leptin and leptin receptor gene(LEPR) polymorphism and patients with breast cancer. Methods LEPR Gln223Arg polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism in 94 patients with breast cancer and 128 healthy controls. The level of leptin were analyzed by enzyme linked immunosorbent assay. Results In univariate regression analyses, we found serum level of leptin and LEPR Gin223Arg genotype polymorphism were significantly higrer than those of the controls (P<0.05-0.001 .respectively). Through multivariable analyses, we found that increased risk estimates for breast cancer were among those with leptin level(OR = 1. 53 ,95% CI: 1.13-2. 07, P = 0. 006), LEPR Gin223Arg genotype(OR = 4.87, 95% CI: 1.30-18.22, P = 0.019), WHR (OR = 3.68,95% CI: 1.34-10.11, P= 0.011). Conclusion Results from this study suggested that LEPR Gln233Agr polymorphism, the elevated WHR and serum level of leptin might be correlated with increased risk of breast cancer.