载脂蛋白B等位基因变异与早发冠心病的关系

目的　研究载脂蛋白 B基因 3 '端 VNTR遗传多态性与早发冠心病之间关系。方法　对 60例早发冠心病患者( CHD)和 5 0例正常对照者 (对照组 )全血 DNA采用聚合酶链反应 ( PCR)法扩增载脂蛋白 B( apolipoprotein B)基因 3 '端可变数目串连重复位点 ( variable num ber tandem ly repeated loci- VNTR)多态性。结果　在 110例中 ,各组的 apo B基因位点呈现了不同的基因带型 ,扩增片段大小为 45 0～ 90 0之间。早发冠心病组发现有 12个等位基因 ,正常人群组有15个等位基因。apo B3 ' 端 VNTR的基因频率分布为 0 .0 0 5～ 0 .3 0 83 ,杂合度为 79.0 %～ 88.8%。早发冠心病组中 ,63 0 bp、660 bp等位基因的频率显著高于正常对照组 ,其血脂水平与对照组有显著性差异 ;不同等位基因组中血脂水平差异无显著性。结论　载脂蛋白 B等位基因 63 0 bp、660 bp可能是早发冠心病的一个危险因子

Relationship between apolipoprotein B gene loci polymorphisms and coronary heart disease

Objective To study the relationship of apolipoprotein B (apoB) polymorphism and coronary heart disease (CHD). Methods The bloodstain DNA was extracted from 110 unrelated individuals of control and 60 with coronary heart disease and their patterns of apoB gene 3'-end VNTR were typed by PCR. Results In 110 individuals,the apoB gene loci of each studied group show different gene band-type ,amplification fragment range in size is 450-900bp.There are 12 apoB VNTR alleles in CHD group, and 15 alleles in the controls. The frequency disrtibution of these 3' apo B VNTR alleles is 0.005-0.3083, a heterozygosity index is 79.0%-88.8%.The fequency of alleles in 630bp,660bp size is significantly higher in CHD than in the controls (P<0.01,P<0.001). Conclusion apoB gene 3'- end VNTR alleles loci 630bp,660bp may be a risky factor of the premature occcurration of coronary heart disease.