| 绿茶表没食子儿茶素没食子酸酯浸提工艺研究 |
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| 引用本文： | 刘元瑞,张杨,赵毅民.绿茶表没食子儿茶素没食子酸酯浸提工艺研究[J].中国药师,2008,11(12). |
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| 作者姓名： | 刘元瑞 张杨 赵毅民 |
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| 作者单位： | 1. 武警部队药品仪器检验所,北京,102613
2. 医学科学院毒物药物研究所 |
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| 摘 要： | 目的：研究绿茶中表没食子儿茶素没食子酸酯（EGCG）的提取工艺，并对不同产地低档茶叶的茶多酚和EGCG的含量进行比较。方法：采用HPLC法测定EGCG含量，以EGCG提取率为考察指标，做单因素试验，再采用正交试验方法，对提取条件进行优化。结果：影响绿茶中EGCG提取效果的主次因素为：乙醇用量〉乙醇浓度〉回流时间〉提取温度。结论：最佳浸提条件为：固液比1：15，以40％乙醇在60℃浸提2次，每次30min，且新茶中EGCG提取率相对较高。
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| 关 键 词： | 表没食子儿茶素没食子酸酯（EGCG） 绿茶 提取工艺 正交设计 |
Study on Extraction Technology of EGCG from the Green Tea |
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| Liu Yuanrui,Zhang Yang,Zhao Yimin.Study on Extraction Technology of EGCG from the Green Tea[J].China Pharmacist,2008,11(12). |
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| Authors: | Liu Yuanrui Zhang Yang Zhao Yimin |
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| Institution: | Liu Yuanrui (Institute for Drug , Instrument Control of the Chinese People\'s Armed Police Forces,Beijing 102613,China),Zhang Yang Zhao Yimin (Institute of Pharmacology , Toxicology,Academy of Military Medical Science) |
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| Abstract: | Objective:To study the extraction technology of EGCG from the green tea and to compare the contents of EGCG and tea-polyphenols in low grade green tea.Method:The EGCG extraction rate was determined by HPLC,EGCE was used as the single factor to find the optimum extractive conditions and than used the orthogonal design.Result:The factors which influence extraction rate was as follows:solid and liquid rtio>amount of alcohol>extration time>temperature,where A,B,C and D stands for the the dosage of ethanol,conce... |
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| Keywords: | EGCG Green tea Extraction technology Orthogonal design |
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