三种方法检测射线照射后细胞存活率的结果比较

目的分析倒置显微镜计数、流式细胞仪及MTT等三种方法检测放射线照射后细胞存活率(S)结果间的差异。方法对相同剂量照射后的同一样本,分别用上述三种方法进行检测及计算以获得S值并进行比较分析。结果三种方法获得的S值结果间差异很大。基本上从流式细胞仪测试结果获得的S值最低,从倒置显微镜计数结果获得的S值次之,而MTT测量结果获得的S值最大,甚至与其他两种方法计算获得的结果有几倍差距。结论在应用上述三种方法进行检测时最好根据实验的具体要求和目的进行选择,否则可能造成结果的偏差。

The Comparison of Cell Survival Probability Values Obtained from Three Methods after Irradiation Respectively

Objective To analyze the difference among the cell survival probability (S) results,which were measured and calculated with microscope, flow cytometry and MTT methods respectively. Methods For the same cell sample after irradiation, the S value was deduced by the above mentioned three methods respectively and was compared with each other. Results Among the S values obtained from three methods, it can be seen that, the lowest one is from flow cytometry, the largest one is from MTT method ,which is so much large as several times as the tolal values of the other two. Conclusion It's better to choose the right method for different experimental objective, otherwise perhaps the error would be caused by the inexact data.