| 平衡透析法、超滤法结合液质联用技术比较测定注射用复方荭草中黄酮类成分的血浆蛋白结合率 |
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| 引用本文： | 黄勇,牟景丽,陈慧,郑林,何峰,张治蓉,王永林. 平衡透析法、超滤法结合液质联用技术比较测定注射用复方荭草中黄酮类成分的血浆蛋白结合率[J]. 中国实验方剂学杂志, 2012, 18(20): 91-96 |
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| 作者姓名： | 黄勇 牟景丽 陈慧 郑林 何峰 张治蓉 王永林 |
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| 作者单位： | 贵阳医学院药学院,贵阳,550004 |
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| 基金项目： | 国家科技重大专项(2008ZX09101-021);国家自然科学基金项目(308603);贵州省科技计划重大专项(黔科合重大专项字[2007]6010号) |
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| 摘 要： | 目的:测定注射用复方荭草中黄酮类成分的人血浆蛋白结合率,为临床安全用药及血浆蛋白测试方法研究提供依据。方法:以超高效液相色谱-质谱联用为检测手段,采用平衡透析法、超滤法考察荭草素、牡荆素、木犀草苷、槲皮苷等黄酮类成分与血浆蛋白的结合情况,并比较二者的差异。结果:注射用复方荭草在人血浆中质量浓度为5～100 mg.L-1时,荭草素、牡荆素、木犀草苷、槲皮苷等黄酮的结合率较强且无浓度依赖性,两种测定方法牡荆素和木犀草苷存在统计学差异(P<0.01)。结论:建立的平衡透析法、超滤法均能够满足血浆蛋白结合率测定的要求,两种方法所测定的部分指标存在差异。
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| 关 键 词： | 黄酮 血浆蛋白结合率 超滤法 平衡透析法 液质联用 |
| 收稿时间： | 2011-12-14 |
Equilibrium Dialysis, Ultrafiltration Combined with UPLC-ESI-MS/MS to Determine the Plasma Protein Binding Rate of Flavonoids in Compound Hongcao Freeze-Dried Powder for Injection |
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| HUANG Yong,MU Jing-li,CHEN Hui,ZHENG Lin,HE Feng,ZHANG Zhi-rong and WANG Yong-lin. Equilibrium Dialysis, Ultrafiltration Combined with UPLC-ESI-MS/MS to Determine the Plasma Protein Binding Rate of Flavonoids in Compound Hongcao Freeze-Dried Powder for Injection[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(20): 91-96 |
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| Authors: | HUANG Yong MU Jing-li CHEN Hui ZHENG Lin HE Feng ZHANG Zhi-rong WANG Yong-lin |
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| Affiliation: | School of pharmacy, Guiyang Medical College, Guiyang 550004, China;School of pharmacy, Guiyang Medical College, Guiyang 550004, China;School of pharmacy, Guiyang Medical College, Guiyang 550004, China;School of pharmacy, Guiyang Medical College, Guiyang 550004, China;School of pharmacy, Guiyang Medical College, Guiyang 550004, China;School of pharmacy, Guiyang Medical College, Guiyang 550004, China;School of pharmacy, Guiyang Medical College, Guiyang 550004, China |
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| Abstract: | |
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| Keywords: | flavonoids plasma protein binding rate equilibrium dialysis ultrafiltration UPLC-ESI-MS/MS |
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