都柏林念珠菌与白念珠菌鉴别研究进展

综述都柏林念珠菌与白念珠菌的鉴别方法。表型比较的方法相对简便易行,可以从芽管、菌落形态及厚壁孢子、产色、荧光、温度、酶活性、碳源同化、红外光谱等多个方面进行鉴别;分子生物学方法可靠性强,包括DNA指纹图、聚合酶链反应、限制性片段长度多态、随机扩增多态DNA、核糖体DNA测序等。

The Update of Identification of Candida dubliniensis from Candida albicans

The approaches to identify Candida dubliniensis from Candida albicans are reviewed. The methods based on phenotypic difference are simple and easy, and the differentiating phenotypes include germ tube formation, colonial morphology, chlamydospore formation, color and fluorescence formation, growth temperature, enzyme activity, carbon source assimilation and infrared spectrum, etc. The molecular biological approaches, which may involve DNA fingerprint, polymerase chain reaction, restriction fragment length polymorphism, random amplification polymorphism DNA, and ribosome DNA sequencing, are more reliable.