乙肝全基因组转基因小鼠肝组织病理及部分免疫学特征

目的:研究C57-TgN(HBVadr2.0) SMMU“3号”品系乙型肝炎转基因小鼠肝脏的组织病理学及部分免疫学特征.方法:通过对130例SPF级乙型肝炎转基因小鼠和30例同龄正常C57BL/6小鼠肝组织进行免疫组织化学染色、HE染色和浸银染色分析,研究转基因小鼠HBsAg的表达以及病理改变情况.选取15例肝组织有单个核细胞浸润的转基因小鼠检测白细胞分化抗原的表达情况.结果:60.77％(79/130)的转基因小鼠肝脏组织中出现了不同程度的肝组织病理性改变,包括肝细胞变性、单个核细胞浸润和纤维组织增生,且与月龄正相关,而与性别、病毒蛋白的表达无相关性.选取出现单个核细胞浸润的肝组织进行免疫组织化学检测发现,肝组织中浸润的单个核细胞大多为CD3+、CD4+T细胞.结论:C57-TgN (HBVadr2.0) SMMU“3号”品系乙肝全基因组转基因小鼠肝脏产生类似慢性无症状HBV携带者的病理学变化,病理改变与病毒蛋白表达无相关性,与小鼠月龄呈正相关性.

Pathological and Some Immunological Characters of Transgenic Mice Harbouring Complete HBV Genomic DNA

Objective: To explore the pathological and some immunological characters of liver in hepatitis B virus(HBV) transgenic mice line C57-TgN(HBVadr2.0) SMMU3.Methods: A total of 130 HBV transgenic mice and 30 C57BL/6 mice were chosen to study the HBsAg expression and pathological changes of liver tissue by immunohistochemical stain,hematoxylin-eosin stain and argyrophilic fiber stain.The expression of leukocyte differentiation antigen in hepatic mononuclear cells from 15 HBV transgenic mice was assayed.Results: Chronic liver histopathological lesions were observed in 60.77%(79/130) transgenic mice as hepatocyte degeneration,mononuclear cells infiltration and fibers hyperplasia,and these changes were correlated with age but not correlated with sex and the expression of virus proteins.Most of the infiltrated hepatic mononuclear cells were CD3+ and CD4+ T lymphocytes by immunohistochemistry detection.Conclusion: It was shown that most of the C57-TgN(HBVadr2.0) SMMU3 transgenic mice developed chronic liver lesion similar to human chronic asymptomatic HBV carrier,and there was positive correlation between chronic liver lesion and age,but no correlation between liver damage and expression of virus proteins.