17β-雌二醇可促进胃癌细胞BGC823生长

目的:观察不同浓度17β-雌二醇(E2β)处理时,人胃癌BGC823细胞生长情况以及雌激素受体ERα36表达的变化,探讨E2 β在胃癌生长调节中的作用及相关机制.方法:BGC823细胞经10-10,10-11和10-12 mol/L浓度的E2β处理24 h和48 h.细胞生长通过WST1方法检测.RT-PCR,Western blot及灰度分析法检测ERα36mRNA水平及蛋白水平变化,其平均光密度值通过t检验分析,应用免疫荧光染色方法检测ERα36蛋白在细胞中的位置变化.结果:E2β可促进胃癌BGC823细胞生长,但随着其浓度的上升,E2β的促生长能力下降.E2β可促进ERα36 mRNA表达,该促进作用具有浓度依赖性.E2β处理BGC823细胞24 h时,ERα36蛋白表达上升,48 h时,ERα36蛋白表达下降.ERα36蛋白定位于细胞膜,E2β对BGC823细胞ERα36蛋白定位无明显影响.结论:E2β可促进胃癌细胞BGC823生长.E2β-ERα36可能通过膜信号通路参与了胃癌细胞的生长调节.

17 Beta-Estradiol Stimulates the Proliferation of Gastric Cancer Cell Line BGC823

Objective: To study the effect of 17β-estradiol(E2β) at different concentrations on the proliferation and estrogen receptor α36(ERα36) expression in gastric cancer cell line BGC823,and to explore the role of E2β in the growth of gastric cancer cells.Methods: BGC823 cells was dealt with 10-10,10-11,and 10-12 mol/L E2β for 24 h and 48 h.Cell growth was assessed by WST1 assay.The mRNA and protein expressions of ERα36 were examined by RT-PCR and Western blot respectively.Average optical densities were detected and processed using Student’s t test analysis.Immuno-fluorescence assay was used to examine ERα36 location in BGC823 cell.Results: E2β could promote the growth of BGC823 cells,but with increasing concentrations,its promoting growth effect decreased.E2β could promote ERα36 mRNA expression in a concentration-dependent manner.E2β could promote ERα36 protein expression in 24 hours,but inhibitory effect was found after 48 hours.The location of ERα36 had no change as treated with E2β.Conclusion: E2β could stimulate the growth of gastric cancer cell line BGC823.E2β-ERα36 may regulate the growth of gastric cancer by membrane signal pathway.