龙胆泻肝汤对PCOS模型大鼠的影响

目的:研究龙胆泻肝汤对多囊卵巢综合征(PCOS)模型大鼠药效观察及机制,为龙胆泻肝汤临床PCOS的应用提供初步实验依据。方法:将SD大鼠50只,随机分为2组,即正常组10只、模型制备组40只,采用胰岛素(INS)联合人绒毛膜促性腺激素(HCG)法复制大鼠PCOS模型,造模成功的大鼠分为模型组、达英-35(0.21 g·kg-1)及龙胆泻肝汤高、低剂量组(29.6,7.4 g·kg-1),除正常组外,其余各组连续灌胃给药15 d;动物处理后测量卵巢质量及体积大小,检测各组大鼠血清中促黄体生成素(LH)/卵泡刺激素(FSH),睾酮(T),空腹血糖,空腹胰岛素(INS)激素水平及炎症因子白细胞介素-2(IL-2),IL-4,IL-6,IL-10水平;蛋白免疫印迹法(Western blot)分析各组卵巢组织中JAK2,STAT3,p-STAT3和IL-6蛋白的表达量。结果:与正常组比较,模型组卵巢质量及体积明显升高,血清LH/FSH,T,空腹血糖及INS均明显升高,IL-2,IL-6,IL-10水平明显升高,JAK2,STAT3和IL-6蛋白的表达量明显升高(P0.05);与模型组比较,龙胆泻肝汤高、低剂量组明显降低卵巢质量及体积,血清LH/FSH,T,空腹血糖及INS,IL-2,IL-6,IL-10水平,升高JAK2,STAT3蛋白的表达量,降低p-STAT3和IL-6蛋白的表达量(P0.05)。结论:龙胆泻肝汤对PCOS大鼠具有明显疗效,主要通过降低激素水平和抑制炎症反应;且其机制可能与JAK2/STAT3通路相关。

Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats

Objective: To study the therapeutic effect and mechanism of Longdan Xiegan Tang on polycystic ovary syndrome (PCOS) model and provide a preliminary experimental basis for the application of PCOS. Method: Totally 50 SD rats were randomly divided into normal group (n=10) and modeling group (n=40). PCOS models were established by using insulin (INS) and human chorionic gonadotropin (HCG). The successfully modeled rats were then divided into model group, Daying-35 group (0.21 g · kg^-1), Longdan Xiegan Tang high-dose group (29.6 g · kg^-1) and low-dose group (7.4 g · kg^-1). After continuous intragastric administration of 15 d, we analyzed the ovarian weight and volume. The levels of luteinizing hormone (LH)/follicle stimulating hormone (FSH), testosterone (T), fasting blood glucose, fasting insulin (INS) and levels of inflammatory cytokines interleukin (IL)-2, IL-4, IL-6 and IL-10; Western blot was used to analyze protein expression of JAK2, STAT3, p-STAT3 and IL-6 in ovarian tissues. Result: As compared with the normal group, ovarian weight and volume were significantly increased; LH/FSH, T, fasting blood glucose and INS levels in serum were significantly increased; IL-2, IL-6 and IL-10 levels were significantly increased; and JAK2, STAT3 and IL-6 protein expression levels were also up-regulated in model group (P<0.05). As compared with the model group, high dose and low dose Longdan Xiegan Tang significantly decreased ovarian weight and volume, LH/FSH, T, fasting blood glucose and INS levels in serum as well as IL-2, IL-6 and IL-10 levels, increased JAK2, STAT3 protein expression and reduced p-STAT3 and IL-6 protein expression (P<0.05). Conclusion: Longdan Xiegan Tang has obvious curative effect on PCOS rats by decreasing hormone level and inhibiting inflammatory reaction and the mechanism may be related to JAK2/STAT3 pathway.