首页 | 本学科首页   官方微博 | 高级检索  
检索        

龙胆泻肝汤对PCOS模型大鼠的影响
引用本文:李俊波,邓芳,李霄汉,许丽绵.龙胆泻肝汤对PCOS模型大鼠的影响[J].中国实验方剂学杂志,2018,24(7):121-126.
作者姓名:李俊波  邓芳  李霄汉  许丽绵
作者单位:广州中医药大学, 广州 510405,广州中医药大学, 广州 510405,广州中医药大学, 广州 510405,广州中医药大学, 广州 510405;广州中医药大学 第一附属医院, 广州 510405
基金项目:广东省科技厅项目(2013A032500016)
摘    要:目的:研究龙胆泻肝汤对多囊卵巢综合征(PCOS)模型大鼠药效观察及机制,为龙胆泻肝汤临床PCOS的应用提供初步实验依据。方法:将SD大鼠50只,随机分为2组,即正常组10只、模型制备组40只,采用胰岛素(INS)联合人绒毛膜促性腺激素(HCG)法复制大鼠PCOS模型,造模成功的大鼠分为模型组、达英-35(0.21 g·kg-1)及龙胆泻肝汤高、低剂量组(29.6,7.4 g·kg-1),除正常组外,其余各组连续灌胃给药15 d;动物处理后测量卵巢质量及体积大小,检测各组大鼠血清中促黄体生成素(LH)/卵泡刺激素(FSH),睾酮(T),空腹血糖,空腹胰岛素(INS)激素水平及炎症因子白细胞介素-2(IL-2),IL-4,IL-6,IL-10水平;蛋白免疫印迹法(Western blot)分析各组卵巢组织中JAK2,STAT3,p-STAT3和IL-6蛋白的表达量。结果:与正常组比较,模型组卵巢质量及体积明显升高,血清LH/FSH,T,空腹血糖及INS均明显升高,IL-2,IL-6,IL-10水平明显升高,JAK2,STAT3和IL-6蛋白的表达量明显升高(P0.05);与模型组比较,龙胆泻肝汤高、低剂量组明显降低卵巢质量及体积,血清LH/FSH,T,空腹血糖及INS,IL-2,IL-6,IL-10水平,升高JAK2,STAT3蛋白的表达量,降低p-STAT3和IL-6蛋白的表达量(P0.05)。结论:龙胆泻肝汤对PCOS大鼠具有明显疗效,主要通过降低激素水平和抑制炎症反应;且其机制可能与JAK2/STAT3通路相关。

关 键 词:龙胆泻肝汤  多囊卵巢综合征(PCOS)  激素水平  炎症因子  JAK2/STAT3通路
收稿时间:2017/11/18 0:00:00

Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats
LI Jun-bo,DENG Fang,LI Xiao-han and XU Li-mian.Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats[J].China Journal of Experimental Traditional Medical Formulae,2018,24(7):121-126.
Authors:LI Jun-bo  DENG Fang  LI Xiao-han and XU Li-mian
Institution:Guangzhou University of Traditional Chinese Medicine(TCM), Guangzhou 510405, China,Guangzhou University of Traditional Chinese Medicine(TCM), Guangzhou 510405, China,Guangzhou University of Traditional Chinese Medicine(TCM), Guangzhou 510405, China and Guangzhou University of Traditional Chinese Medicine(TCM), Guangzhou 510405, China;First Affiliated Hospital of Guang zhou University of TCM, Guangzhou 510405, China
Abstract:Objective: To study the therapeutic effect and mechanism of Longdan Xiegan Tang on polycystic ovary syndrome (PCOS) model and provide a preliminary experimental basis for the application of PCOS. Method: Totally 50 SD rats were randomly divided into normal group (n=10) and modeling group (n=40). PCOS models were established by using insulin (INS) and human chorionic gonadotropin (HCG). The successfully modeled rats were then divided into model group, Daying-35 group (0.21 g · kg-1), Longdan Xiegan Tang high-dose group (29.6 g · kg-1) and low-dose group (7.4 g · kg-1). After continuous intragastric administration of 15 d, we analyzed the ovarian weight and volume. The levels of luteinizing hormone (LH)/follicle stimulating hormone (FSH), testosterone (T), fasting blood glucose, fasting insulin (INS) and levels of inflammatory cytokines interleukin (IL)-2, IL-4, IL-6 and IL-10; Western blot was used to analyze protein expression of JAK2, STAT3, p-STAT3 and IL-6 in ovarian tissues. Result: As compared with the normal group, ovarian weight and volume were significantly increased; LH/FSH, T, fasting blood glucose and INS levels in serum were significantly increased; IL-2, IL-6 and IL-10 levels were significantly increased; and JAK2, STAT3 and IL-6 protein expression levels were also up-regulated in model group (P<0.05). As compared with the model group, high dose and low dose Longdan Xiegan Tang significantly decreased ovarian weight and volume, LH/FSH, T, fasting blood glucose and INS levels in serum as well as IL-2, IL-6 and IL-10 levels, increased JAK2, STAT3 protein expression and reduced p-STAT3 and IL-6 protein expression (P<0.05). Conclusion: Longdan Xiegan Tang has obvious curative effect on PCOS rats by decreasing hormone level and inhibiting inflammatory reaction and the mechanism may be related to JAK2/STAT3 pathway.
Keywords:Longdan Xiegan Tang  polycystic ovary syndrome (PCOS)  hormones  inflammatory cytokines  JAK2/STAT3 pathway
本文献已被 CNKI 等数据库收录!
点击此处可从《中国实验方剂学杂志》浏览原始摘要信息
点击此处可从《中国实验方剂学杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号