| 经腹腔转染iNOS基因对大鼠的降血压作用 |
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| 引用本文： | 曾秋棠,黄恺,祝武强,廖玉华. 经腹腔转染iNOS基因对大鼠的降血压作用[J]. 中国病理生理杂志, 2002, 18(4): 381-384 |
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| 作者姓名： | 曾秋棠 黄恺 祝武强 廖玉华 |
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| 作者单位： | 华中科技大学同济医学院心血管病研究所, 湖北武汉 430022 |
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| 摘 要： | 目的:研究经腹腔转染人iNOScDNA进入正常大鼠体内对大鼠血压的影响及转染的iNOS基因在其体内表达的情况。方法:经腹腔基因转染。结果:①经腹腔转染iNOS基因大鼠的血压从转染后第2d起明显较注射前降低,至第6d降低仍有显著差异(P＜0.01);②转染iNOS基因大鼠在转染后第3d、第6d腹腔巨噬细胞的NO水平明显高于对照组(P＜0.01)。结论:经腹腔转染iNOS能明显降低大鼠血压。
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| 关 键 词： | 血压 转染 一氧化氮合酶 基因 |
| 文章编号： | 1000-4718(2002)04-0381-04 |
| 收稿时间： | 2000-11-03 |
| 修稿时间： | 2000-11-03 |
Effect of iNOS gene transfection via enterocoelia on blood pressure in rats |
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| ZENG Qiu-tang,HUANG Kai,ZHU Wu-qiang,LIAO Yu-hua. Effect of iNOS gene transfection via enterocoelia on blood pressure in rats[J]. Chinese Journal of Pathophysiology, 2002, 18(4): 381-384 |
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| Authors: | ZENG Qiu-tang HUANG Kai ZHU Wu-qiang LIAO Yu-hua |
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| Affiliation: | Institute of Cardiovascular Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | AIM: To investigate the effect of human iNOS gene transfection on blood pressure in SD rats. METHODS: Human iNOS cDNA was transfected using gene transfection technique via enterocolia. The iNOS gene expression in rat celiac macrophages was also observed. RESULTS: The significant lowering of blood pressure in rats transfected with iNOS gene was observed from second day to sixth day after transfection, compaered to the control group ( P<0.01 ). The NO level in transfected rat celiac macrophages at sixth day after transfection was singnificantly higher than that in control group.CONCLUSION: The results indicated that method of gene transfer via enterocolia may be useful for the hypertension therapy. |
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| Keywords: | Blood pressure Transfection Nitric-oxide synthase Gene |
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