A gel electrophoretic analysis of proteins from plants infected with tobacco mosaic and potato spindle tuber viruses |
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| Authors: | M Zaitlin V Hariharasubramanian |
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| Affiliation: | 1. Computational Bioscience Research Center, King Abdullah University of Science and Technology, 4700 KAUST, Thuwal 23955-6900, Saudi Arabia;2. Graduate School of Information Science and Technology, Hokkaido University, N14W9 Kita, Sapporo 060-0814, Japan;3. Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan;4. Department of Population Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan;5. Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Shizuoka 411-8540, Japan;6. Department of Fish Ecology and Evolution, EAWAG, Swiss Federal Institute of Aquatic Science and Technology, 6047 Kastanienbaum, Switzerland;7. Research Faculty of Agriculture, Hokkaido University, N9W9 Kita, Sapporo 060-8589, Japan;1. Department of Public Health Sciences, College of Health Sciences, University of Texas, El Paso, USA;2. Border Biomedical Research Center, Toxicology Core, University of Texas, El Paso, USA;3. Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY, USA;4. Department of Psychology, University of Texas, El Paso, USA |
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| Abstract: | The relative incorporation of leucine-3H or -14C into proteins of virus-infected and healthy leaf tissue was used to detect virus mediated or virus-stimulated protein synthesis. Proteins from subcellular fractions of tobacco mosaic virus (TMV)-infected and potato spindle tuber virus (PSTV)-infected tissue were analyzed by electrophoresis in sodium dodecyl sulfate containing polyacrylamide gels.With TMV infections in tobacco, incorporation of leucine was stimulated into 4 proteins of molecular weights of 245, 195, 155, and 37 × 103 daltons (d) and into the coat protein monomer of 17.5 × 103 d. The predominant new or stimulated protein, found in all subcellular fractions was the 155 × 103 d component; the “nuclear” and “mitochondrial” fractions showed considerable stimulation into all 5 proteins. The “mitochondrial” fraction prepared from leaves infected with the defective TMV strain PM2I also showed stimulated incorporation into both the 155 × 103 d component and the nonfunctional coat protein.In extracts of PSTV-infected tomato plants no proteins of a molecular weight expected for a virus coat protein were detected, lending support to the conclusion that no protein-containing virions are produced by this unusual virus. However, incorporation of leucine was stimulated in two proteins which were approximately the size of the 155 and 195 × 103 d TMV-induced proteins. |
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