白细胞介素-6基因多态性与2型糖尿病肾病的关联性研究

目的　研究白细胞介素 6 (IL 6 )基因启动子区 6 34C/G多态性与 2型糖尿病肾病的关系。方法　运用聚合酶链反应—限制性片段长度多态性 (PCR RFLP)方法检测了 2 6 7例 2型糖尿病患者 ,其中正常白蛋白尿者 90例 ,微量白蛋白尿者 85例 ,大量白蛋白尿者 92例及 12 6例非糖尿病对照者的IL 6基因型。结果　①大量白蛋白尿组GG基因型频率和G等位基因频率高于正常组及正常白蛋白尿组 ,有统计学差异 (均P <0 .0 5 )。②微量白蛋白尿组与正常组和正常白蛋白尿组相比 ,基因型和等位基因频率均无显著性差异 (均P >0 .0 5 )。结论　IL 6基因 6 34C/G多态性可能是糖尿病肾病进展的一个遗传易感因子。

Association of IL-6 Gene Polymorphism with Diabetic Nephropathy in Type 2 Diabetes

Objective The aim of this paper is to investigate the association of IL-6 gene -634C/G polymorphism in the promoter region with diabetic nephropathy in type 2 diabetes. Methods IL-6 genotype was determined by PCR-RFLP in 267 type 2 diabetes patients(90 with normoalbuminuria,85 with microalbuminuria and 92 with macroalbuminuria) and 126 non-diabetic controls. Results The frequency of the GG genotype and G allele significantly increased in the patients with macroalbuminuria compared with that in patients with normoalbuminuria and the control group(both P<0.05). Compared with patients with normoalbuminuria and the control group, IL-6 genotype frequency and allele frequency in the patients with microalbuminuria were not statistically significant (both P>0.05). Conclusion IL-6 gene-634C/G polymorphism may be a genetic susceptibility factor for the progression of diabetic nephropathy.