Cognitive dysfunctions and spontaneous EEG alterations induced by hippocampal amyloid pathology in rats |
| |
| Affiliation: | 1. Department of Drug Science and Technology, Turin University, Corso Raffaello 30, 10125, Torino, Italy;2. Akdeniz University Faculty of Medicine Department of Biophysics, Antalya, Turkey;1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Biochemistry, School of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran;3. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran;1. Department of Dermatology, The First Medical Center of PLA General Hospital, Beijing, China;2. Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical College, Qiqihar City, Heilongjiang Province, China;3. Department of Dermatology, Hainan Hospital of PLA General Hospital, Sanya City, Hainan Province, China;1. Department of Pediatric Surgery and Urology, Medical University of Bialystok, Bialystok, Poland;2. Department of Neurosurgery, Medical University of Bialystok, Bialystok, Poland;3. Department of Biophysics, Medical University of Bialystok, Bialystok, Poland;4. Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland;5. Department of Invasive Neurology, Medical University of Bialystok, Bialystok, Poland;1. Department of Nephrology, Dialysis and Internal Diseases, Medical University of Warsaw, Warsaw, Poland;2. Department of Anaesthesiology and Intensive Care, Medical University of Warsaw, Warsaw, Poland;3. Department of General, Liver and Transplantation Surgery, Medical University of Warsaw, Warsaw, Poland;4. Department of Medical Informatics and Telemedicine, Medical University of Warsaw, Warsaw, Poland;1. Department of Hematology, Medical University of Bialystok, Bialystok, Poland;2. Department of Allergology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland;3. Department of Experimental Allergology and Immunology, Medical University of Bialystok, Bialystok, Poland;1. Department of Medicine, Division of Gastroenterology and Hepatology, Shinshu University School of Medicine, Matsumoto, Japan;2. Department of Radiology, Shinshu University School of Medicine, Matsumoto, Japan;3. Department of Internal Medicine, Matsumoto Dental University, Shiojiri, Japan |
| |
| Abstract: | PurposeWe aimed to determine the effects of different doses of amyloid-beta (Aβ) peptide on learning and memory, and whether the changes in brain oscillations induced by dose-dependent accumulation of Aβ could be used as biomarkers to detect early stages of Alzheimer's disease (AD).Material and methodsMale albino Wistar rats aged 3 months were randomly divided into four groups (n ?= ?12/group) obtained by i. h. Injection (to the dorsal hippocampus) of saline or different doses of 0.01 ?μg/μl, 0.1 ?μg/μl, and 1 ?μg/μl of Aβ. After two weeks of recovery period, open field and novel object recognition tests were performed and spontaneous EEG recordings were obtained. Later, hippocampus tissues were collected for Western blot and ELISA analysis.ResultsA significant decrement in recognition memory was observed in 0.1 ?μg/μl, and 1 ?μg/μl injected groups. In addition, Aβ accumulation induced significant decrement of the expression of NeuN, SNAP-25, SYP, and PSD-95 proteins, and led to the increment of GFAP expression in hippocampus. Moreover, we detected remarkable alterations in spontaneous brain activity. The hippocampal Aβ levels were negatively correlated with hippocampal gamma power and positively correlated with hippocampal theta power. Also, we observed significant changes in coherence values, indicating the functional connectivity between different brain regions, after the accumulation of Aβ. Especially, there was a significant correlation between changes in frontohippocampal theta coherence and in frontotemporal theta coherence.ConclusionsOur findings indicate that Aβ peptide induces AD-like molecular changes at certain doses, and these changes could be detected by evaluating brain oscillations. |
| |
| Keywords: | Amyloid beta Synaptic proteins Electroencephalography Behavior Rat |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
