汉族、哈萨克族湿热痹阻证类风湿关节炎患者Wnt信号通路的研究

目的:检测汉族、哈萨克族湿热痹阻证类风湿关节炎患者Wnt信号通路基因,以期发现不同民族类风湿关节炎患者在Wnt信号通路上的表现机制是否存在差异,为进一步研究类风湿关节炎的发病机制及临床治疗提供理论依据。方法:运用PCR Array技术对4例哈萨克族和4例汉族湿热痹阻证类风湿关节炎患者的Wnt基因进行检测,筛选出表达差异有统计学意义且与类风湿关节炎发病相关的基因,再选取哈萨克族(试验组)与汉族(对照组)湿热痹阻证类风湿关节炎患者,每组30例,应用实时荧光定量PCR技术对其外周血进行检测,进一步验证所筛选出的Wnt基因表达差异。结果:试验组与对照组相比,有21个Wnt基因表达差异有统计学意义(差异倍数>2,P<0.05)。从中选出FZD9、MMP7、Wnt8A、Wnt1和Axin2基因,用实时荧光定量PCR技术进一步验证,发现这5个基因在试验组中均呈现高表达,2组间Wnt8A、Axin2和Wnt1比较,差异有统计学意义(P<0.05)。结论:哈萨克族与汉族湿热痹阻证类风湿关节炎患者相比,Wnt8A、Axin2和Wnt1基因存在显著差异,且呈正向倍数变化,这些基因的异常表达可能与哈萨克族类风湿关节炎患者发病特点有关。

Wnt Signaling Pathway in Patients with Rheumatoid Arthritis of Dampness-heat-bi-obstruction Syndrome among the Han and Kazak Nationalities

Objective:To detect Wnt signaling pathway gene in patients with rheumatoid arthritis of dampness-heat-bi-obstruction syndrome among the Han and Kazak nationalities,in order to find out whether there are differences in the expression mechanism of Wnt signaling pathway among different nationalities,and provide theoretical basis for further research on the pathogenesis and clinical treatment of rheumatoid arthritis.Methods:PCR Array technology was used to detect the Wnt gene of four Kazak patients and four Han patients with rheumatoid arthritis of dampness-heat-bi-obstruction syndrome.Another sixty patients(thirty of each nationality)were selected as the experiment group(Kazak)and the control group(Han),and their peripheral blood was detected by real-time fluorescence quantitative PCR to further verify the expression difference of the screened Wnt gene.Results:Compared with the control group,the expression of twenty-one Wnt genes in the experiment group was significantly different(difference multiple>2,P<0.05).Five genes,i.e.FZD9,MMP7,WNT8A,WNT1 and AXIN2 were selected and further verified by real-time fluorescence quantitative PCR.It was found that these five genes were highly expressed in the experiment group.There were statistically significant differences between the two groups in WNT8A,AXIN2and WNT1(P<0.05).Conclusion:There are significant differences in WNT8A,AXIN2and WNT1 genes between Kazak and Han patients with rheumatoid arthritis of dampness-heat-bi-obstruction syndrome,and the abnormal expression of these genes may be related to the pathogenesis of Kazak patients with rheumatoid arthritis.