DNA barcoding of hard ticks (Ixodidae), notes on distribution of vector species and new faunal record for Croatia |
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| Institution: | 1. Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek 31000, Croatia;2. Department of Epidemiology, Andrija ?tampar Teaching Institute of Public Health, Zagreb 10000, Croatia;3. Faculty of Forestry and Wood Technology, University of Zagreb, Zagreb 10000, Croatia;4. Department of Biology, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia;5. Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb 10000, Croatia;6. Department of Epidemiology, Teaching Institute of Public Health Istria County, Pula 52100, Croatia;7. Molecular Biology Division, Ru?er Bo?kovi? Institute, Zagreb 10000, Croatia;1. Institute of Chemical Biology and Fundamental Medicine, SB RAS, prosp. Lavrent''eva 8 630090, Novosibirsk, Russian Federation;2. Novosibirsk State Medical University, Novosibirsk, Russian Federation;1. Paris-Saclay University, AgroParisTech, INRAE, JRU MIA-Paris, 75005, Paris, France;2. Université Clermont Auvergne, AgroParisTech, INRAE, VetAgro Sup, JRU Territoires, F-63170 Aubière, France;3. Université Clermont Auvergne, CNRS, LMGE, F-63000, Clermont-Ferrand, France;4. Université de Lyon, INRAE, VetAgro Sup, UMR EPIA, F- 69280 Marcy l''Etoile, France;5. Université Clermont Auvergne, INRAE, VetAgro Sup, UMR EPIA, F-63122 Saint-Genès Champanelle, France;6. Université Clermont Auvergne, CHU Clermont-Ferrand, CNRS, LMGE, F-63000 Clermont–Ferrand, France;1. Department of Entomology, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, CT 06511, USA;2. Center for Vector Biology & Zoonotic Diseases, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, CT 06511, USA;3. Department of Epidemiology of Microbial Diseases, Yale School of Public Health, 60 College Street, P. O. Box 208034, New Haven, CT 06520, USA;4. Department of Forestry and Horticulture, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, CT 06511, USA;5. United States Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Diagnostics and Biologics, National Veterinary Services Laboratories, 1920 Dayton Avenue, Ames, IA 50010, USA;1. Laboratory of Parasitology, Department of Disease Control, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Japan;2. Division of Bioinformatics, International Institute for Zoonosis Control, Hokkaido University, Hokkaido 001-0020, Japan;3. Department of Animal Medicine, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt;4. Department of Parasitology, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia;5. School of Veterinary Science, University of Queensland, Gatton Qld, 4343, Australia;6. Division of Risk Analysis and Management, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan;7. International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan;8. One Health Research Center, Hokkaido University, Sapporo 060-0818, Japan;9. School of Science, Technology and Engineering, GeneCology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland 4558, Australia;10. Epidemiology, Parasites and Vectors, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort 0110, South Africa;11. Department of Life and Consumer Sciences, University of South Africa, South Africa;12. Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria 0110, South Africa;1. Department of Animal Pathology, Faculty of Veterinary, Lugo. University of Santiago de Compostela, Spain;2. Department of Veterinary Services, Kenya Wildlife Service, Nairobi, Kenya;3. Department of Biochemistry and Molecular Biology, Egerton University, Njoro, Kenya;4. International Livestock Research Institute, Nairobi, Kenya;5. Department of Biology, Duke University, Durham North Carolina, USA;6. Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Sevilla Spain;7. Wildlife Research and Training Institute, Naivasha, Kenya |
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| Abstract: | Molecular methods are increasingly being utilized for accurate identification of ticks (Acari: Ixodidae), especially in cases of morphologically highly similar species. In this study, we performed molecular research of the tick fauna in Croatia using DNA barcoding method. Ticks were sampled in three biogeographical regions and thirteen species were recorded: Dermacentor marginatus, Dermacentor reticulatus, Haemaphysalis concinna, Haemaphysalis inermis, Haemaphysalis punctata, Hyalomma marginatum, Ixodes frontalis, Ixodes hexagonus, Ixodes kaiseri, Ixodes ricinus, Rhipicephalus bursa, Rhipicephalus sanguineus s.l. and Rhipicephalus turanicus. Ixodes kaiseri is for the first time recorded in the fauna of Croatia. Of the thirteen hard tick species analyzed in this study, pathogens from different groups (bacteria, protozoa and viruses) have been detected in eight species in Croatia so far. For the important vector species R. sanguineus s.s., new distributional data for Croatia are given. The standard COI barcoding region was amplified, and the sequences were analyzed by species delimitation methods together with the sequences of conspecific and congeneric species from the public BOLD database. Our specimens of H. punctata represent a new, genetically distinct MOTU. A brief overview of the available public DNA barcoding data for Ixodidae is presented, highlighting the need for an integrative approach for the clarification of the taxonomic status of problematic Ixodid taxa. The results provide a basis for the establishment of a molecular data platform for the Ixodidae of the Croatian fauna. |
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