Serotonin may alter the pattern of gonadotropin-induced progesterone release of human granulosa cells in superfusion system |
| |
Authors: | Koppan Miklos Bodis Jozsef Verzar Zsofia Tinneberg Hans-Rudolf Torok Attila |
| |
Institution: | Department of Obstetrics and Gynaecology, Baranya County Teaching Hospital, Univ of Pécs, Faculty of Health Sciences, H-7623 Pécs, Rakoczi Str. 2, Hungary. mkoppan@freemail.hu |
| |
Abstract: | Serotonin plays a hormonal function in several nonneuronal peripheral tissues, such as the ovaries. Our aim was to investigate
whether there is a modulatory action of serotonin on gonadotropin-induced steroid secretion of human granulosa cells. In granulosa
cell culture, serotonin was administered alone or in combination with luteinizing hormone (LH) and follicle-stimulating hormone
(FSH). Also, granulosa cells were transferred into a dynamic superfusion apparatus and challenged by FSH and LH alone or along
with serotonin. Estradiol and progesterone concentrations of samples were measured by radioimmunoassay. As expected, administration
of FSH, LH, and serotonin alone resulted in a significant estradiol and progesterone release in cell culture, as well as a
significant increase in progesterone release in dynamic superfusion system. In cell culture, co-administration of serotonin
with gonadotropins had no additive effect on gonadotropin-induced secretion of progesterone, while it further augmented that
of estradiol. In superfusion system, when gonadotropins were added along with serotonin, the increase in progesterone release
was markedly less, while peaks of hormone response were remarkably prolonged compared to challenges by LH and FSH alone. The
observed effects of serotonin on gonadotropin-induced steroid release of granulosa cells may reveal further details about
the regulation of granulosa cell function. |
| |
Keywords: | Serotonin LH FSH granulosa cell superfusion cell culture estradiol progesterone |
本文献已被 PubMed SpringerLink 等数据库收录! |
|